| Oryza officinalis is one of the native wild rice in our country,which contains many excellent genetic traits,and is also an important rice germplasm resource in China.In this research,we analyzed the transcriptome data of the wild rice seedlings under high temperature and salt stress,the results were as follows.The total of 30.28 Gb Clean Data were obtained by sequencing transcriptome of three samples under the high temperature,high salt stress and control,the Clean Data of each sample reached 3.08 Gb,and the percentage of Q30 base was above 86.06%.A total of93339 transcript and 50233 unigenes were obtained from 7701307 conting sequences produced by transcriptome sequencing.The number of transcript with the length greater than 1000 is 41159,accounting for 44.09% of the total number of transcript.The number of unigenes with length greater than 1000 is 14304,accounting for 28.55% of the total number of Unigene.The N50 length of transcript and unigene were 1878 bp and 1443 bp,respectively.The total of 26139 unigenes were annotated in GO database and accounted for 52.04%of the total number of unigenes.Among them,the number of unigene with length greater than 300 bp is 21979,accounting for more than 62.16% of the total number of unigenes.The number of unigene with length greater than 1000 bp is 11589,accounting for 81.19% of the total unigenes over 1000 bp.The largest number of unigenes were assigned to two subtypes(catalytic activity)for the catalytic activity and binding(binding)of molecular function annotation,the proportion was more than 50% of the total.In cellular components annotation,three subtypes,including cell part,organelle and cellcell,ranked first three list,and each proportion were significantly more than 10%.In biological process annotation,the top three subtypes(cellular process)were cellular pathway,metabolic pathways and a single body process.The total of 15180 unigenes were well annotated by KOG database,which areclassified into 25 functional categories.A total of 3106 unigenes were classfied into general function of KOG database,accounting for 20.46% of the total unigene annotated in KOG.Secondly,the unigenes,involving in the function class of posttranslational modification protein turnover,chaperoneswere,listed second place and consisted of a total of 1705 unigene,accounting for 11.23% of the total unigenes.The third category was signal transduction mechanism,the percentage was 10.65%.The remaining unigenes were grouped into the categories,including sugar transport and metabolism,unknown function class unknown),secondary metabolite biosynthesis,transport and catabolism,amino acid transport and metabolism and transcription,the percentage of unigene were 6.57%,5.58%,5.48%,5.36% and 5.30%,respectively.There were 2057 differentially expressed genes under high temperature stress,1004up-regulated and 1053 down regulated,indicating that there were no significant differences in the amounts of up-regulated and down regulated genes under high temperature stress.Compared with the control group,there were 272 up-regulated genes and 8 down regulated genes in the high salt stress group.High salt stress up-regulated genes are mainly high abundance of transcription factor zinc finger,ap2,Myb and WRKY family,high temperature stress up-regulated genes are mainly zinc finger,Hsp,Myb,WRKY,sugar transporter.In order to verify the accuracy of transcriptome sequencing under the high salt stress transcriptome sequencing results and high temperature stress,serval unigenes with different expression change were selected to validate,using real-time quantitative PCR method,the fold changes of unigenes were consistent with those fo transcriptome sequencing results,indicating that transcriptome sequencing results was credible. |
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