Genetic Background Study Of Macaca Mulatta Using STR And The Genetic Variation Characteristics Of A Closed Macaca Mulatta Colony

Background:Macaca mulatta is a Cercopithecinae primate.They are distributed in the Southwest,South,North of China,as well as India,Bangladesh,Nepal,and Vietnam,and are important experimental non-human primates.Macaca mulatta with clear genetic background are the basis for the construction of animal models,and they are also the guarantee of the accuracy of biomedical research results.STR(Short Tandem Repeat)is a co-dominant molecular genetic marker with a high degree of polymorphism.It is mainly used for studying population genetic background.Objective:Acquire STR distribution characteristics of Macaca mulatta in Institute of Medicial Biology Chinese Academy of Medicial Science,to provide Macaca mulatta with clear genetic background for scientific research.Methods:A total of 24 STR loci in 58 rhesus monkeys were detected by fluorescence-labeled PCR and capillary electrophoresis.The data was processed and analyzed using software such as GeneMapper,CERVUS,and Popgene.Results:The number of alleles per locus ranged from 4 to 20.The average expected heterozygosities of popl and pop2 were 0.7373 and 0.7555,respectively;the mean observed heterozygosities were 0.7125 and 0.7566,respectively;the average Shannon information indices were 1.5548 and 1.7029,respectively.The average number of alleles for POP1 was 7.13 and POP2 was 9.25.The Hardy Weinberg equilibrium test results of 24 STR loci showed that 11 sites in popl and pop2 deviated from Hardy-Weinberg equilibrium,and 7 sites were the same,which were D6S276,D6S291,D6S1691,D6S2741,D11S2002,DRA-CA,D6S2876;4 different sites,pop1 for MICA,D6S501,D5S1457 and D4S2365,pop2 for D10S611,D15S823,D19S255 and DXS2506.UPGMA cluster analysis showed that the Chinese-sourced rhesus monkeys were clustered in one and separated from Indian sources.Nei's genetic differentiation coefficient calculation results showed that the total genetic diversity(HT)was 0.735,the average genetic diversity(Hs)within the population was 0.747.Conclusions:The 24 STR loci differed in the distribution of alleles in selected rhesus monkeys,and there was no regular distribution,the polymorphism was high.The genetic diversity indices of both groups are at a high level.There are 11 STR loci in popl and pop2 deviating from the Harvard equilibrium.We speculate that the direct cause of this phenomenon is that the number of rhesus monkeys selected for this study was too small.The results of Nei's genetic diversity parameters showed that the group diversity mainly comes from within the population.The UPGMA phylogenetic tree showed that the Chinese rhesus macaques and the Indian rhesus macaques were in different branches and the genetic distance was far away,indicating that there were differences in the genetic background between the Chinese rhesus monkeys and the Indian rhesus monkeys.Background:CCR5 is the major co-receptor for M-tropic HIV-1 and Simian immunodeficiency virus infecting target cells.Rhesus Monkey is a good animal model for studying human immunodeficiency virus.Therefore,studying the mutation of CCR5 gene in rhesus monkeys and the differences between rhesus monkey CCR5 gene and human may provide useful information for revealing the pathogenesis of HIV and SIV,understanding the development of AIDS,and the genetic treatment of AIDS.information.Objective:To study the mutation of CCR5 in rhesus monkey.Compare the similarities and differences in the nucleic acid sequence and amino acid sequence between the rhesus monkey and human CCR5 gene,and to explore the differences between these sites and their functions.Methods:A total of 82 Rhesus Monkeys were randomly selected for the PCR amplification and sequencing of their whole CCR5 gene sequence.Alignment and comparison of CCR5 was performed with DNASTAR and MEGA.Results:Compared with the GenBank Rhesus monkey DNA reference sequence,there were 8 mutation sites in the CCR5 gene sequence of Rhesus monkeys,all of which were synonymous mutations,of which 3 sites were identical to the human CCR5 gene mutation.To compare with human DNA reference sequence in GenBank revealed that a total of 33 sites were different in base,8 of them encoded different amino acids.Conclusions:The CCR5 gene mutation in rhesus monkeys can be used as a good model to study the role and mechanism of CCR5 gene mutation in SIV infection.