Construction Of Microfluidic Organ-on-a-chip And Three-Dimensional Culture Technique Of Bone Microvascular Endothelial Cells

Organ-on-a-chip is a micro cell culture device that simulates the function of human organ unit in vitro using microfluidic chip as the core technology,organ chip can be used to build disease models,replace animal experiments in the process of new drug development,exercise powerful screening functions and many other uses.There are now different types of organ-on-a-chip and even human-on-chips,the huge potential they have shown has also been valued by society,government and industry in recent years,there is no doubt that organ chips have very broad application prospects and industrialization capabilities.Nontraumatic osteonecrosis of femoral head is a common refractory disease in orthopedics,in bone repair,the formation of vascular granulation tissue and the proliferation of fibrous connective tissue are particularly important.Combined with the design of microfluidic chip to simulate the skeletal angiogenesis in vivo involved in the vascular germination in mineralized bone tissue,it is very important to construct a femoral head necrosis model.This subject constructs a microfluidic organ-on-a-chip,which is successfully constructed by PDMS casting optimization,second chip modification,and chip function verification series of steps.Then 3D culture of BMEC is carried out in the chip,and it can be observed under the microscope that BMEC grows into a microvascular network in the organ chip under the co-culture of fibroblasts and HA.Finally,the micro vascular structure in the chip was treated with glucocorticoids and icariin.The stimulating damage of glucocorticoids to the microvascular structure and the protective effect of icariin were observed.The construction of the BMEC microvascular structure in the microfluidic organ-on-a-chip has successfully achieved the microvascularization of the in situ vascular endothelial cells of the femoral head in the organ chip.It is closer to the real bone microvessel appearance in the human body and more realistically simulates the human ischemia microvessel growth and germination during repair of osteonecrosis provide an in vitro model for ischemic osteonecrosis.The model can be used to observe the effect of drugs on the morphology and growth of microvessels in ischemic osteonecrosis,and can be used to screen drugs and study the pathogenesis of osteonecrosis.