3D Circular Human Body Bionic Enteric Chip With Intestinal Villus Structure And Intestinal Peristalsis

There are a large number of bacteria in the human intestine,which are normal microorganisms in that.These bacteria are called intestinal flora.Some studies indicated that the intestinal flora is intricately related to the occurrence of tumors,and it can also significantly affect the efficacy of some oral anti-tumor drugs,but the relationship has not been fully elucidated.The existing methods for studying intestinal flora mainly include two methods of using a sterile mouse to transplant a human flora and performing a macro genome detection on a patient's intestinal flora,which have their own defect that results are different from the human body and it is difficult to carry out harmful interference experiments.In order to effectively study the relationship between intestinal flora and tumors.This study combines the use of 3D printing technology and surface microstructure transfer technology to produce the surface microstructure chip.Compared the difference between culturing cells on resin 3D chips and culturing cells on polydimethylsiloxane(PDMS)chips.The surface coverage and cell density of the cells on the chip were studied under various conditions such as chip surface modification,microstructure shape and height,and culture time.According to the size of the human intestine,we creatively fabricated a human intestinal chip similar to the shape of the small intestine in human body and having a small intestine villi structure.After the cells were added into the chip,they were rotated by a rotation speed of 2 rpm for 4-6 hours,and it was found that cells were attached to each side of the chip after a 5 days culture.After exploring the relationship between cell height and density on the chip and time,it is appropriate to carry out subsequent experiments on the 5th day.In the intestinal chip,a simulation of human small bowel peristalsis is achieved by a soft material for the chips and an external mechanical structure.After the intestine chip simulating the villus in vivo was set up in vitro,in this chip,we realized the in vitro co-culture of intestinal bacteria and intestinal cells in 24 hours by the combination of the villus structure,the liquid flow and the peristalsis.It was also found that peristalsis during co-culture can effectively reduce the area of bacterial attachment and better maintain the state of the cells.The chip realized culturing strict anaerobic intestinal bacteria in an anaerobic environment.It provides a new research platform and method for the subsequent study of intestinal flora and the correlation between flora and tumor.