Study On Chemiluminescence Enhancement Behavior Of Peroxyoxalate Based On Aggregation-induced Emisson Molecules

Chemiluminescence(CL)is a phenomenon of light emission that occurs during a chemical reaction.Compared with other luminescence methods,chemiluminescence analysis does not require an external light source.There is no interference of Rayleigh scattering and Raman scattering,and it overcomes the disadvantages of signal fluctuation caused by instability of the light source,thereby improving the signal-to-noise ratio.Coupled with the application of high-sensitivity photoelectric detection technology,the chemiluminescence analysis method has the advantages of high sensitivity,wide linear range,simple instrumentation,rapid analysis,and easy automation.There are many factors affecting the behavior of chemiluminescence resonance energy transfer:the spectral energy overlap of the donor and the acceptor,the distance between donor and acceptor in the progress of chemiluminescence resonance energy transfer,the concentration of the reactants,the contact area,the fluorescence properties of the acceptor and so on.In this thesis,the TPE-AOT molecule was designed and synthesized firstly,which is a surfactant with aggregation-induced emission property.And We used the static-injection chemiluminescence analysis method to study the influencing factor that can improve the chemiluminescence(CL)Intensity of peroxyoxalate systems(TCPO),such as microemulsion,aggregation-induced emission molecule and the distance between the donor and acceptor in the process of resonance energy transfer(CRET).The main research content of the paper is as follows:(1)A surfactant molecule with aggregation-induced emission properties was designed and synthesized:TPE-AOT.And it can form an optical,transparent homogeneous microemulsion with a bright green fluorescence,which can sensitize the heterogeneous chemiluminescence reaction system.Since the distance between the energy donor and the acceptor plays a crucial role in the progress of chemiluminescent resonance energy transfer(CRET),and the distance between the traditional chemiluminescence donor and acceptor is uncontrollable,so,We designed a light-emitting analogue of di-isooctylsuccinate sodium sulfosuccinate(AOT)with Aggregation Induced Emission(AIE)properties(expressed as TPE-AOT)to control the distance between the donors and acceptors at 1.1 nm.So,it can be used as a CRET receptor.(2)The second part is to study the effect of the distance between the donor and the receptor in the CRET progress to the CL intensity in the media of TPE-AOT microemulsion.The prepared TPE-AOT microemulsion is an optically transparent homogeneous solution with a bright green fluorescence.And,it can fix the position of the peroxyoxalate chemiluminescence(CL)donor on the oil-water boundary and achieve peroxygen peroxide.The controllable distance betwreen the acid donor is 1.14 nm,and the TPE-AOT receptor significantly increased the CRET efficiency(93.4%).However,in the traditional microemulsion and fluorescent dye chemiluminescence systems,CRET efficiency is lower(59.01%).In addition,the detection limit of H2O2 in TPE-AOT microemulsion can reach 0.77 ?M,one order of magnitude lower than the traditional microemulsion staining system.And TPE-AOT and TCPO were modified into water-soluble nanoparticles for the detection of H2O2,which is expected to be applied to the detection of H2O2 in cells.