Stduy Chemical Constituents Of Gentiana Macrophylla Pall And Their Anti-inflammatory Activities

Gentiana macrophylla Pall belongs to the genus of Gentianaceae plant,with complex chemical constituents,mainly including iridoids,flavonoids,xanthones,alkaloids,triterpenoids and other compounds.Some of these compounds exhibited cholagogic and liver-protective,anti-inflammatory analgesic,antibacterial,antioxidant,antiviral,anti-tumor,central neural and immunomodulatory effect.In this thesis,the roots of G.macrophylla Pall were selected to investigate their chemical composition.The roots were extracted with ethyl alcohol/water,then the crude extract was suspended in water and partitioned with chloroform to provide the chloroform extract,successively,the same procedure was conducted with n-butyl alcohol instead of chloroform to provide the n-butyl alcohol extract.The chloroform extract were studied using a series of column chromatography separation methods together with modern spectroscopic technologies(NMR,MS,UV).Fifteen compounds,including erythrocentaurin,2-methoxyanofinic acid,gentioxepine,melissic acid,gentianine,butylparaben,erythricin,N-tetracosanoylanthranilic acid,N-pentacosanoylanthranilic acid,N-hexacosanoylanthranilic acid,N-heptacosanoylanthranilic acid,N-octacosanoylanthranilic acid,Roburic acid,caffeic acid and β-sitosterol,were isolated from the roots of G.macrophylla Pall,among which N-pentacosanoylanthranilic acid and N-heptacosanoylanthranilic acid were elucidated as two new compounds,which providing more aboundant compounds source for the chemical substances based research of G.macrophylla Pall.Compounds and crude extract isolated from G.macrophylla Pall were evaluated with the anti-inflammatory research through using the model of mice abdominal cavity macrophage RAW 264.7 with inflammation reflection induced by Lipopolysaccharide(LPS).The inhibitory rates of NO were regarded as a indicator in this model.Result showing that the chemical constituents isolated from G.macrophylla Pall exhibited significant anti-inflammatory activities and the anti-inflammatory activity increased with higher sample concentration.