Study Of PEGylated Fluorescent Supramoleculer System For Drug Delivery

With the rapid development of biomedicine,the exploration of new anticancer methods has become one of the focuses of scientific attention in recent years.Due to the growing incidence of cancer in modern society,the means of prevention and treatment of cancer are increasingly diversified.The current treatments of cancer are the chemotherapy-based traditional means,meanwhile,there are other new treating methods including radiotherapy,surgical resection,gene therapy,photodynamic therapy.Chemotherapy has its convenience and effectiveness,but the bad side effects of chemotherapy on patients also become a problem that plagues clinical medicine,and gene therapy is limited by contemporary medical conditions.Photodynamic therapy is a new anti-cancer treatment with less trauma and suitable for local treatment,which make it raised more attention.Due to the high water content in the human body and the majority of photosensitizer drugs in the present stage of photodynamic therapy are hydrophobic,it is necessary to construct a safe,hydrophilic,and pharmacologically acceptable Drug carriers,to achieve a variety of treating methods for improving the cure rate of cancer.In this thesis,we combined the fluorescence chemistry with supramolecular chemistry to design and synthesize a polyethylene glycol-modified bis-bromopyrromethene Boron Difluoride(BODIPY).The photo-sensitizer backbone compound Mi was synthesized mainly from bis-bromopyrromethene Boron Difluoride and the hydrophilic-modified side-chain compound M2 was synthesized from polyethylene glycol monomethyl ether(mPEG).Finally,M1 and M2 were condensed to form the final target compound M3.In addition,we also designed and synthesized a new type of aggregation-inducing fluorescent probe M4 and studied its recognition performance against hydrogen sulfide.The structures of the synthesized intermediates and target compounds in this dissertation were confirmed by 1H-NMR,13C-NMR and MS.The final target compound M3 contains a long-chain supramolecular long chain with the main structure of mPEG The compound containing this structure is hydrophilic and is a good water-soluble substance that can be used as a drug carrier for drug transport in the blood.Compound M3 containing Dibromo-BODIPY core structure with photosensitivity can be used as a good photodynamic therapy agent.Based on this study,the performance of the water-soluble photosensitized supramolecular material in both photodynamic therapy and on anti-cancer drug delivery was investigated.This study combines both drug transport and photodynamic therapy products,providing a new combined sufficiently approach to cancer treatment.Each step of synthesis has high feasibility and the synthetic yield of each step is good.In addition,the target product has entered the testing phase of biological activity.