Study On Quality Standard Of Yizhiningshen Powder

With the development of science and technology,each subject has a breakthrough on their original basis,the chemical analysis technology has made great progress,which has been widely applied in the evaluation of the quality standard of traditional Chinese medicine.The progress and application of chemical analysis technology make the quality standards of traditional Chinese medicine has been improved to a great extent.In this paper,the qualitative and quantitative quality standard research comprehensively launched on traditional Chinese Medicine yizhiningshensan,makes it conform to the efficient quality control.Provide a strong basis for quality approval of hospital preparations and effective control of the preparation.This paper takes advantage of a thin layer chromatography can be qualitative identification of traditional Chinese medicine,using this method to launch qualitative analysis of yizhiningshensan.Through the research to different kind of thin layer plate different methods,different developing agent and detection wavelength.The analysis method is highly specific qualitative with the advantage of simple operation and well reproducibility,and this provides a method for efficient,accurate analysis of qualitative of yizhiningshensan.Qualitative identification of Radix Astragali:According to this method,prepare the preparation of test materials,control article,control medicine,the control solution,apply silica gel G plate,with chloroform and methanol and water(13:7:2),getting solution as developing agent which placed lower below 10℃,then spread,remove,dry,and spray with 10%suLfuric acid ethanol solution which was used as chromogenic agent,then heating up to 105℃,so as to spot color clear,and viewing it under the UV lamp(365nm).Qualitative identification of Radix ginseng:According to this method,prepare the preparation of test materials,control article,apply silica gel G plate,with chloroform and methanol and water(13:7:2),getting solution as developing agent which placed lower below 10 ℃ then expansion,remove,dry,and spray with 10%suLfuric acid ethanol solution which was used as chromogenic agent,then heating up to 105℃,so as to spot color clear,and viewing it under the UV lamp(365nm).Qualitative identification of Radix Ophiopogonis:According to this method,prepare the preparation of test materials,the reference solution,apply silica gel G thin plate,with chloroform and ethyl acetate and acetone(8:1:1),getting solution as developing agent,expansion,remove,dry,and spray with 10%suLfuric acid ethanol solution,the color clear heating at 105℃,so as to spot color clear,and viewing it under the UV lamp(365nm).Qualitative identification of Radix Angelicae Sinensis:According to this method,prepare the preparation of test materials,control article,control medicine,the control solution,apply silica gel G thin plate,with cyclohexane and dichloromethane and ethyl acetate and formic acid(4:1:1:0.1),getting solution as developing agent,expansion,removed,dry,and viewing it under the UV lamp(365nm).Qualitative identification of Radix Bupleuri:According to this method,prepare the preparation of test materials,control artcle,control medicine,the reference solution,apply silica gel G plate,with chloroform and methanol and water(13:7:2),getting solution as developing agent,expansion,remove,dry,and spray with 2%vanillic acid 40%suLfuric acid(V:V)solution,and viewing it under the UV lamp(365nm).High performance liquid chromatographythe can determine the particuLar herb in traditional Chinese Medicine,In this paper,using this method,determine the Astragalus in yizhiningshensan.Applying Thin layer chromatography to study yizhiningshensan on qualitative identification,getting clear discrimination diagram,and have good degree of separation,which can prevent the interference of negative control effectively,this method has strong specificity.Apply High performance liquid chromatographythe determine the Astragalus in yizhiningshensan,the determination of astragaloside in Radix Astragali is content.The resuLts showed that astragaloside is in 2.5～15 μg range,and display good linear relationship,the linear regression equation is Y=1.162X+11.888,R=0.9996.precision test showed RSD=1.18%,reproducibility showed RSD=2.07%,stability showed RSD=1.17%,recovery of the added sample showed RSD=2.77%,the determination of sample showed RSD=2.79%,the average recovery rate showed 93.73%.