| Algae is the most widely distributed light-autotrophic organism on the earth.It is an important provider of oxygen in the atmosphere.Algae have a short growth cycle but a large biomass.Some algae have strong adaptability to the environment and contain pigments and oils.,protein and other active substances.Therefore,algae is widely used in many fields such as aquatic products,food,medicine,agriculture,energy,and environmental protection.To ensure long-term preservation of the genetic stability of selected single species of microalgae,to maintain rare and endangered microalgae germplasm resources,to facilitate exchange of germplasm in academic exchanges,to facilitate the selection of excellent germplasm,and to save manpower during production.We studied the Isochrysis galbana,Chlorella sp.,Microcystis sp.,Tetraselmis and the laboratory preservation of Hainan University.Cryopreservation of five algal species of the genus Alexamdrum sp..In the experiment,the use of an encapsidation-dehydration method and ice-binding protein was successfully cryopreserved by Isochrysis galbana,Chlorella sp.and Microcystis cultivated in the laboratory of Hainan University.sp.),Tetraselmis,Alexandium sp.Five algal species,and innovative research frozen ice and antifreeze protein as a frozen reagent to change the toxicity of the past embedded microalgae with cryotoxic reagents The disadvantages of injury to experimental operators provide a scientific theoretical basis for the development and use of microalgae cryopreservation special cryogenic reagents.Encapusulation-dehyration method was applied to preserve five different microalgal species,including Alexandium sp.,Isochrysis galbana,Chlorella sp..,Tetraselmis sp.,and Microcystis sp..During early stationary phase,the algal cells were encapsulated in 6%Na-alginate beads with 3%CaC12.The ice-biding proteins(IBPS)were added into the reagents.This study assessed the effect of dehydration rate,beads water content,and sucrose concentration on the survival rate of five algal species.This research showed that,(1)The optimal dehydration rate of beads was 1.1%/h.(2)The optimal-beads-water.content of Isochrysis galbana was 33.4%.The optimal water content of other algal beads were 40.4%.(3)There were no interactive effects between dehydration rate and water content of the beads.(4)The survival rate had been improved significantly after pre-culturing.(5)Without adding IBPS,the survival rates of Alexandium sp.,Isochrysis galbana,Tetraselmis sp.,Chlorella sp..,and Microcystis sp..were 27.4%29.6%33.5%34.9%54.2%However after adding IBPS,the survival rates of these five algal species were 34.7%36.8%45.7%52.7and 72.7%The survival rates of all five species were improved by adding IBPS while applying Encapsulation-dehydration method.By adding IBPS,encapsulation-dehydration method became more efficient and more applicable to preserve algal species with a great amount.Therefore,encapsulation-dehydration with IBPS method would have a wide range of application prospects in the medium-term conservation of microalgae.The new protective agent ice-associated antifreeze protein IBPS during embedding-dehydration reduces the production of ice crystals during cryopreservation and prevents ice crystal regeneration during the rapid temperature-rising dissolution process,resulting in high resistance of marine microalgae cells.Freezing force improves the preservation effect and reduces the possibility of genetic variation.This operation is simple and easy,without complicated and expensive equipment,the use of non-toxic antifreeze protection agent,can make Isochrysis galbana,Chlorella sp.,Microcystis,unique cell wall in Haikou.Five species(Microcystis sp.),Tetraselmis,and Alexamdrum sp.were successfully... |
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