Separation And Purification Of Ellagic Acid Of And Its Effect On Lipid Peroxidation In Lonicera CaeruleaL.

The experiment targets on ellagic acid of Lonicera caeruleaL.,it has optimized the extraction and has specified the condition of separation and purification.Qualitative and quantitative analysis of ellagic acid of Lonicera caerulea were identified by high performance liquid chromatography.Lipid peroxidation and the degradation of ellagic acid in simulated gastric intestinal juice was then studied,The main findings are as follows:The ellagic acid in Lonicera caerulea were extracted by using Solvent extraction and Ultrasonic.Ultrasonic extracting method is elected as the optimal approach according to their impact on productivity.The Lonicera caerulea powder and 80%acetone-water mixed solution were mixed at the ratio of 1:33(g/mL).Supersonic extraction was carried out at ultrasonic power of temperature of 51℃ for 54min,and the yied of the ellagic acid in Lonicera caerulea was 18.31mg/g.The separation and purification of ellagic acid were studied.The experiment shows that purification has a significant effect on the purity of ellagic acid.HPD-600 was elected from five kinds of macroporous to carry out the first extraction and purification of ellagic acid:the static adsorption lasted for 6h,taking 50%ethanol solution as the eluent,with 3h desorption time;The concentration of the sample was controlled at 2.5mg/mL,the flow rate was 3mL/min,and the injection volume 50mL,then elution was carried out with 60%ethanol solution.And then the second separation and purification was carried out with High efficiency adsorption chromatography resin CG-161:the static adsorption lasted for 3.5h,25℃ was set as the best adsorption temperature,and taking 13%sodium hydroxide-60%ethanol solution as eluent;When the most favorable sample volume was 40mL,elution was carried out at 40mL of eluent with a concentration 60%,and the flow rate was 3mL/min.The purity ellagic acid of was 35.26%,61.07%and 80.18%respectively.The component identification of ellagic acid was identified by high performance liquid chromatography.Bycomparing with the high performance liquid chromatogram of standard product,the content of ellagic acid was 80.07%.It can be determined that the main ingredient in Lonicera caerulea is ellagic acid.Anti-lipid peroxidation activity of Ellagic acid and in vitro simulation of gastrointestinal digestion model are also studied.The results showed that the content of ellagic acid was significantly changed after gastric and intestinal digestion,increased by 36.5%and 36.87%respectively.It is proved that Lonicera caerulea can release more ellagic acid in gastrointestinal tract digestion.The in vitro anti-lipid peroxidation experiments showed that ellagic acid had inhibitory effect on lipid peroxidation induced by spontaneous and H2O2 or Fe2+.And the inhibition of spontaneous lipid peroxidation in liver tissues of rats was most obvious.Moreover,the Anti-lipid peroxidation of ellagic acid purified liquid was stronger than that of crude extract.Within a certain concentration range,The anti-lipid peroxidation activity increased with the increase of ellagic acid concentration.When ellagic acid concentration was 600 g/mL,the anti-lipid peroxidation activity of ellagic acid in Lonicera caerulea reached a maximum value of 83.59%.