Development Of A Rapid Detection Equipment For Multi-blood Samples Based On Fluorescent Immune Chromatography Technique

Fluorescent immune chromatography technique is a rapid quantitative detection technique based on fluorescence immunoassay technique and immune chromatography technique,which can obtain the concentration of specific substance according to quantitative arithmetic,making use of the fluorescence signal produced by fluorescent markers under the excitation light.It has some advantages such as good stability,strong specificity and high sensitivity.The technique has been widely used in the domain of medicine,environment and biology,especially when it comes to the diagnosis for bacterial and virus infectious diseases,it has an important clinical value.After analyzing the quantitative detection based on fluorescent immune chromatography,this paper designed rapid detection equipment for multi-blood samples according to the function requirements and completed the commissioning work,with various disciplines knowledge of mechanics,optics,electronics and software engineering.Through the coordination work of multiple motors and different sensors,the equipment implemented a series of operations,such as initialization,sample injection,pushing reagent strips,washing,detecting and throwing rejected material away.Photoelectric detection module adopted double closed-loop feedback circuit,ensuring the stability of excitation light from LED.Dual-channel light path structure was designed to realize the concentration detection for C-reactive protein(CRP)and procalcitonin(PCT)of blood samples.The expected intensity values could be acquired by analysis of the fluorescence intensity scanning curve,through peak-searching algorithm and filter algorithm.In order to improve the quantity precision of sample adding,and clean the sampling needle during the process,a corresponding fluid circuit was designed.Besides,the circuit and software of low machine control system were developed based on the chip STM32,realizing bottom control for the equipment.Human-computer interactive interface was designed using the development platform QT on Linux operation system,and the upper machine communicated with the lower machine through a serial port according some communication protocol.Finally,preliminary tests were implemented for the equipment performance such as detection precision and repetition accuracy,after the system unit debug.The equipment developed in this paper have high precision and stability in detections of CRP and PCT,totally reaching the design requirement.It can avoid the error brought by artificial process of adding samples and greatly improve the detection efficiency for multiple blood samples with high degree of automation.