Studies On Purification And Antioxidant Activity Of Brazil Mushroom Polypenols

Agaricus blazei Murill is a new raw material for health care products.Researchers at home and abroad have found that its active ingredients have the functions of preventing and treating diabetes,anti-oxidation and anti-cancer.In Europe,the United States,Japan and other places,related health food products have been gradually developed.And listed,so the health care value of Agaricus blazei is increasingly concerned by people.However,most studies of Agaricus blazei at this stage focus on polysaccharides,glycoproteins,nucleic acids,lipids,and sterols.Agaricus blazei polyphenols have a relatively low content,and there are only a few reports in the literature.Polyphenols have good anti-oxidation,hypolipidemic,hypoglycemic and other effects;while Agaricus blazei has a large production volume and short growth cycle,which is conducive to the extraction and development of polyphenols.Based on the potential development and application value of Agaricus blazei polyphenols,the aim of this study is to establish an extraction process suitable for industrial production of Agaricus blazei Murrill polyphenols and purification process of macroporous adsorption resin,and to establish an efficient and stable high-speed countercurrent preparation method for polyphenolic monomers.The chemical composition of polyphenols lays the foundation for further research on the active function of Agaricus blazei polyphenols and product development.The main contents of the study are as follows:Through single-factor experiments and orthogonal experiments,the stable conditions for extracting Agaricus blazei Murrill polyphenols were determined: Agaricus blazei Murill powder was dried at 60 mesh,solvent water was extracted,and the solid-liquid ratio of 1:20 was leached once in a 90 °C water bath.,extract 1 h.Comparing six different resins,combined with the adsorption capacity and resolution,the NKA-2 resin was selected as the column chromatography chromatographic separation material of Agaricus blazei Murrill,and the purification process conditions were optimized.The optimum conditions were selected.The results were: pH 3,loading concentration 0.32 mg/mL,loading volume 5 BV,loading flow rate 4 BV/h,elution solvent 80% ethanol,elution volume 6 BV,elution flow rate 3 BV/h.According to this process condition,the elution rate of Agaricus blazei polyphenols was 85.00%,and the content of polyphenols in the extract was 22.08%,which enabled the accumulation of polyphenols of Agaricus blazei.The crude extract of Agaricus blazei Murill with NKA-2 resin was used as raw material,using water-methanol-n-butanol-glacial acetic acid(4:1:5:0.05V/V)and water-methanol-ethyl acetate-glacial acetic acid.(4:1:5:0.05V/V)is a solvent system.The three main compounds in Agaricus blazei Murrill were obtained through two separations.The purity was 92%(component 1)and 95%(components)by liquid chromatography.2),97%(component 3).The crude extracts and resin products of Agaricus blazei Murrill and the components 1 and 3 separated by high-speed countercurrent flow were subjected to simple antioxidant experiments using the salicylic acid method and the Prussian blue method.The crude extracts and resin products of Agaricus blazei were obtained by comparison.The Vc contents of component 1 and component 3 separated by high-speed countercurrent flow were 0.32,0.50,0.65,and 0.45 mg,respectively;the hydroxyl radical scavenging rates were 54.9%,79.4%,88.7%,and 63.3%,respectively.Electrospray Ionization Mass Spectrometry(ESI-MS)was used to analyze the two polyphenols separated by high-speed countercurrent flow.Epicatechin and quercetin were detected in negative ion mode.