The environmentally harmless utilization and disposition of antibiotic bacterial residue has become an urgent issue that needs to be solved for industry and government management.In order to effectively realize the resource utilization of antibiotic bacterial residue,realize the dual purpose of bacteria residues treatment and economic benefit creation.The SEA-CBS high-efficiency composite resource utilization technology has been developed to treat antibiotic bacteria residues,bacteria residue fertilizer is made after harmless treatment and resource utilization.However,it remains to be studied whether the technology is feasible,whether the prepared bacterial residue organic fertilizer has valuable applications in agriculture,whether the small amount of residual antibiotics poses environmental risks in the soil and are toxic to crops,etc.Therefore,this experiment take neomycin sulfate residue fertilizer as the research object,investigat its influence on the greenhouse soil environment and the growth of chicory,and provide scientific basis for the agricultural value evaluation and safe utilization of neomycin sulfate residue fertilizer.The main experimental results obtained are as follows:In order to study whether it is feasible to treat antibiotic residue with SEA-CBS technology,the physicochemical properties of neomycin sulfate residue fertilizer produced under three different technological conditions were tested.The test results show that the moisture content,pH,heavy metal content,organic matter and total nutrient content of the bacterial residue fertilizer produced under the three different technological conditions all met the requirements of "Biological Organic Fertilizer"(NY 884-2012)and "Organic Fertilizer"(NY525-2012).This shows that the SEA-CBS technology can be used to treat antibiotic dregs to make dregs fertilizer.The results showed that it is feasible to make bacterial residue fertilizer by SEA-CBS technology.1)The bacteria residue fertilizer under the second process is selected to study the effect of different quality bacteria residue fertilizer on the soil environment.The results show that after the antibiotic bacteria residue is treat harmlessly,the microbial biomass and antibiotic resistance gene abundance were significantly reduced.2)The bacteria residue fertilizer under the second technological conditions is selected to study the effect of different quality bacteria residue fertilizer on the soil environment.The results show that the microbial biomass and antibiotic resistance gene abundance of antibiotic residue were significantly decreased after the harmlessly treated.During the application of bacterial residue organic fertilizer,the relative abundance of the neomycin sulfate resistance gene acc(6 ’)ib in soil increased,but it is significantly lower than that of commercial organic fertilizer.The larger the proportion of bacterial residue organic fertilizer application,the higher the enzyme activity of soil.It can be seen that the application of bacterial residue organic fertilizer is conducive to improving soil enzyme activity,promoting the conversion and absorption of nutrients in the soil,and increasing soil fertility.When the application amount of neomycin sulfate bacterial residue organic fertilizer is increased from 1% to 3%,the growth performance of chicory is improved.The effect of neomycin sulfate bacterial residue organic fertilizer on the growth of chicory is better than that of commercial organic fertilizer.3)The ecological risk assessment of the application of neomycin sulfate bacterial residue organic fertilizer into soil showed that neomycin sulfate have been completely degraded in the process of fertilizer application and is harmless to terrestrial ecosystem.The aquatic ecological risk assessment of the application of neomycin sulfate bacterial residue organic fertilizer indicated that the ecological risk level is very low when the aquatic ecological risk characterization value of neomycin sulfate bacterial residue is far less than 0.1. |