Hydrogen Production By Combined Fermentation Of Excess Sludge And Cyanobacteria And Construction Of Hydrogen Fuel Cell

With the rapid economic development and the increase in energy demand,renewable energy has received widespread attention.Hydrogen is one of the clean and renewable energy sources,and its research has become a hot field.The traditional hydrogen production technology is costly,and will cause secondary pollution to the environment during the hydrogen production process.The anaerobic fermentation hydrogen production technology uses microbial metabolism to convert organic matter into hydrogen without pollution to the environment,while obtaining clean energy.Effectively absorb organic solid waste pollutants,and realize the reduction.harmlessness and recycling of their treatment.In this paper,the typical algae spirulina in surplus sludge and cyanobacteria is the research object.First,the pretreatment method(heat treatment,acid treatment,alkali treatment),pretreatment time and solid-liquid ratio design three-factor three-level orthogonal experiment are selected to study The effect of different spirulina pretreatment methods on the yield of reducing sugar was optimized to obtain the best spirulina pretreatment method;secondly,the anaerobic fermentation was carried out by combining spirulina and excess sludge.By analyzing the amount of hydrogen production,the rate of hydrogen production,Liquid-phase end products of fermentation substrates and microbial community structure,explore the effects of different mixing ratios,different initial pHs,and different fermentation temperatures on hydrogen production from combined fermentation,optimize the conditions of hydrogen production from combined fermentation,and provide fuel for hydrogen fuel cells to study hydrogen Fuel cell power generation characteristics.The results showed that different pretreatment methods had certain influence on the production of reducing sugar by spirulina,among which alkali treatment had the best effect.When the pH of pretreatment was 11.0,the pretreatment time was 24 hours,and the solid-liquid ratio was 1:20,the production of reducing sugar by Spirulina was the largest,which was 2.34mg/L.Hydrogen production experiment was carried out by combining excess sludge with Spirulina after alkali pretreatment.The results showed that when the mixing ratio was 1:4,the hydrogen production and hydrogen production rate were 111.1 mLH2 and 22.66 mLH2/g·TS;when the initial pH was 8.0,the hydrogen production and hydrogen production rate were 125 mLH2 and 25.52 mLH2/g·TS;when the fermentation temperature was 40?,the hydrogen production and hydrogen production rate were 112.31 mLH2 and 22.92 mLH2/g·TS,respectively.The response surface method was used to optimize the fermentation conditions.The influence degree of the three factors on the hydrogen production was the mixture ratio>initial pH>fermentation temperature.Acetic acid and butyric acid are the main end products of fermentation liquid.The higher the ratio of butyric acid to acetic acid is,the higher the hydrogen production efficiency is.The hydrogen produced by anaerobic fermentation is used in the power generation module for power generation.The measured maximum voltage is 1.1107V.When the external resistance is 710 ? and the voltage on both sides of the battery is 0.866V the power density of the battery is the maximum,which is 0,375mW and 599.76mw/m2.