Preparation And Characterization Of Anti-infectious/inflammatory Functionalized Tissue Repair Materials

Tissue repair includes wound closure and tissue regeneration.Tissue repair materials have already been widely utilized in clinic,aiming at regenerating novel tissue in vivo both structurally and functionally.However,infectious/non-infectious inflammation would lead to tissue repair failure,and even threat patients lives.Aiming at solving this infectious/non-infectious inflammation problem,functionalizing tissue repair materials with anti-microbial/inflammatory factors and studying its mechanism could provide novel ideas.Therefore,this research is of scientific significance.Thus,this thesis proceeded following research results:1.SIS degradation products were produced by in vitro enzyme degradation,with its molecular weight concentrated among 10 ~ 17 kDa and strong anti-microbial property.Prepared by coating/freeze-drying method,pSIS/COL I composite freezedrying porous scaffolds were equipped with these strong anti-microbial factors and formed a special structure of “functionalized porous coating layer infiltrating the original pores”.These composite scaffolds were of good biocompatibility.The cocultured MG-63 cells could normally adhere,grow,with their typical morphology remained and proliferation rate promoted by 42% in co-culture time d 3.2.pSIS/SIS composite tissue repair patches were prepared by coating/freezedrying method and therefore successfully equipped with strong anti-microbial property and their hydrophilicity significantly improved as well.The SIS patches were of a certain micro morphology of orientated collagen sheaf and therefore,the pSIS coating distributed along this collagen sheaf orientation.The composite patch was of good biocompatibility.The co-cultured MG-63 cells could normally adhere and proliferate with their typical morphology remained.Implantation experiments and histological analysis showed that no hydatoncus or inflammation was spotted and native cells could infiltrate,adhere,proliferate and excrete ECM in the composite patches,indicating their good histocompatibility.3.Taxifolin-loaded PHBV fibrous membrane was prepared by physical blending and electrospinning with its fiber diameter among 0.91 ~ 1.01 ?m.Under the disturbance of IL-1?,the morphology of the co-cultured chondrocytes had transformed from their normal typical ellipse plum filopodia form to abnormality of strip-like spindle shape with fibrosis,hypertrophy,and their filopodia shrink into fiber-like shape.Chondrocytes co-cultured with 0.04 wt% and 2.5 wt% taxifolin-loaded fibrous membranes showed certain such morphology abnormality but meanwhile,certain amount of cells still stood their normal typical morphology and meanwhile their proliferation rate promoted.The chondrocyte typical-morphology-related gene SOX9 of the 0.04 wt% taxifolin-loaded fibrous membrane experimental group was also proven to be promoted.All of the biological experiments indicated that taxifolin-loaded PHBV fibrous membranes had chondrocyte morphology protecting and proliferation promoting effects under the IL-1? model.In summary,in this thesis,anti-infectious/inflammation factors functionalized tissue repair materials were proven to be able to show their antimicrobial/inflammation properties.The researches further access the biocompatibility or tissue compatibility of the composite tissue repair materials,aiming at provide theoretical bases for the clinical application of anti-infectious/inflammation tissue repair materials.What's more,taxifolin-loaded PHBV electrospun fibrous films were prepared and their influence on tissue repair process was studied.The results indicated that traditional Chinese medicine bioactive factor taxifolin could enhance chondrocytes activity under inflammation environments.