Study The Dynamic Trans-membrane Transport And Mechanism Of Gold Nanocages

Gold nanocages(Au NCs),as a nanomaterial,have attracted much eye-catching in the fields of photothermal therapy(PTT)and drug delivery(DDS).Studying the dynamic processes of Au NCs of different sizes is one of the key steps to explore the efficiency of PTT and DDS.Au NCs trans-membrane transport is a dynamic process driven by force.Studying the dynamic process of Au NCs trans-membrane is of great significance for screening suitable nano-based drugs based on Au NCs.In this paper,the force tracing technology based on atomic force microscopy(AFM)is used to analyze the trans-membrane transport process of a single Au NCs under physiological conditions.The dynamic parameters of the transport process of Au NCs of two sizes are determined and the differences between the two are analyzed.Simultaneously,the two techniques of force tracing and confocal fluorescence microscope imaging were used to study the trans-membrane pathway.The research progress is as follows:(1)Recording the kinetic process of single 50 nm single particle gold nanocage transmembrane transport,and measure the force,time,displacement and velocity required for the 50 nm gold nanocage transport trans-membrane.The results show that during the trans-membrane transport process,the 50 nm gold nanocage requires an average internal force of 77.2±1.34 pN;the corresponding internal time is 184.97±5.62 ms;its displacement is 29.76 nm;the average velocity is 0.161 μm/s.(2)Measuring the trans-membrane transport kinetic parameters(force,time,displacement and velocity)of a single 100 nm gold nanocage.The average endocytosis value required for 100 nm gold nanocage transport trans-membrane is 101.71±2.01 pN;the corresponding endocytosis time is 426.63±20.93 ms;its displacement is 31.09 nm;the average velocity is 0.0728μm/s.The kinetic parameters of 50 nm Au NCs and 100 nm Au NCs trans-membrane transport were analyzed and compared,in which 50 nm Au NCs trans-membrane transport requires less force,shorter time and faster speed.(3)The force tracing technique and the fluorescence confocal microscope imaging technique were used to study the trans-membrane transport pathway of Au NCs of two sizes.The guided endocytosis and giant cell drink are completed in synergy.