Study On Application Of Sensor Array Based On Perylene Derivatives In Food Detection

In recent years,due to the frequent occurrence of food safety accidents and the serious consequences,food safety has become one of the hottest issues in the world.As a result,chemical components detection in food is an important link for food quality and safety control.At present,multiple analytical methods have been developed,but these traditional methods have disadvantages and limitations such as time-consuming,requiring large equipment,and high-cost.As an emerging technology which mimics the mammalian olfactory system,sensor array has advantages of simple,efficient construction procedures and low-cost.In recent years,sensor array has been widely used to discrimination and detection of various substances such as metal ions,proteins,bacteria,and foods.In this paper,three novel fluorescence sensor arrays based on perylene derivatives are constructed and applied to the discrimination of metal ions and proteins in food samples.(1)Based on the metal ions induced perylene derivatives self-assembly,a novel fluorescence sensor array was successfully constructed to discriminate multiple metal ions and different brands of drinking water.Two functionalized perylene probes(Pery-1 and Pery-2)were employed in the work,which could interact with metal ions via electrostatic and coordinate bonding interactions.As a result,monomer emission was quenched,and excimer emission was observed.Depending on the metal ions used,various degrees of quenching of monomer emission and generation of excimer emission were observed.Based on the above principles,a sensor array was constructed to successfully discriminated 11 metal ions and quantitatively detect the metal ions.The array was also successfully applied to the differentiation of 9 different brands of commercial mineral water,and the evaluation of drinking water quality.(2)Based on the polymer induced perylene derivatives self-assembly,a novel fluorescence sensor array was constructed and successfully applied to the identification of milk adulteration.A carboxyl modified negatively charged peryleneprobe was employed to construct the array.In a neutral buffer solution,the probe existed in its monomeric form.Addition of cationic polymer induced the aggregation of the negatively charged probe due to electrostatic interaction and resulted into large quenching of monomer emission and generation of excimer emission.Afterwards upon addition of negatively charged protein,monomer emission restored with a decrease in excimer emission due to interaction of negatively charged protein with cationic polymer.Based on the above principles,a sensor array was developed and successfully discriminated 5 major proteins in milk.In addition,we successfully identified pure milk out of different drinks.Furthermore,the array exhibited excellent performance to discriminate milk adulterated by different concentrations of adulterants,and showed the ability for quantitative detection of milk adulteration.(3)Based on the single-stranded DNA(ssDNA)induced perylene derivatives self-assembly,a novel fluorescence sensor array was successfully constructed to discriminate multiple proteins.A positively charged perylene probe(PeryProbe)was employed.In the presence of negatively charged ssDNA,PeryProbe aggregated which caused probe fluorescence quenching.After the addition of a protein,the protein could interact with the assembled ssDNA-PeryProbe complex with change of fluorescence signals.Based on the above principles,a sensor array was constructed and successfully discriminated 9 proteins.And the proteins could also be clearly recognized in human urine sample.