Preparation Of Aspergillus Niger Fungal Mycelium Pellets Using Ramie Degumming Wastewater And Loading Oms-2 To Degrade Dye

Dye wastewater is one of the main sources of environmental pollution.Biological adsorption method has been widely concerned in the treatment of dye wastewater.This adsorption technology has the characteristics of feasibility,flexibility and simple operation.However,the industrial large-scale use of biological adsorption technology to treat dye wastewater has a problem of high cost.At the same time,the adsorption effect of biological materials on dye wastewater is selective and limited,resulting in the large-scale use of biological adsorption technology is still in a stagnation state.The application of nano-catalytic materials in the field of dye wastewater treatment has also become one of the hotspots of researchers.This paper hopes to degrade the dye wastewater that is difficult to adsorb by combining biological adsorption materials with nano-catalytic materials.Therefore,this paper uses the degumming waste liquid produced in the process of ramie biological degumming as a medium to prepare the biomass carrier material mycelium ball as the carrier of the nanocatalytic material OMS-2.The OMS-2/mycelium composite material with catalytic function strengthens the application of nano-catalyst materials in the treatment of dye wastewater,and at the same time reduces the cost consumption in the application process.In this thesis,the ramie biological degumming waste liquid was used as a culture medium,and Aspergillus niger fungus spores were cultured under different conditions.Through characterization observation,the experimental conditions were continuously optimized,and finally the best preparation conditions were obtained,and intact mycelium balls were cultivated.Then the mycelium pellet was used to assemble OMS-2 under non-nutritive conditions.The assembly process used the mycelium pellet as a unique self-assembly ability of the organism.A series of characterization tests were performed on the assembled composites.Characterization was performed using XRD,FT-IR,SEM and other methods for structural and morphological analysis.By using dye X-3B as a pollution source to simulate wastewater degradation experiments,the application effect of OMS-2/hyphae in dye wastewater treatment was explored.The results showed that the ramie biological degumming solution can be used as a medium for Aspergillus niger spores and prepare hyphae.The culture conditions are neutral alkali,the rotation speed is 120 rpm,and the culture is performed at 37 °C for 72 hours to obtain mycelium pellets.The prepared mycelium pellets are washed with deionized water to complete selfassembly under the same culture conditions for 12 hours.This process successfully assembles nanomaterials into functional materials with macrostructures.SEM analysis showed that OMS-2 was uniformly loaded on the surface of mycelium,and the morphology was good.XRD and FT-IR analysis showed that did not change the structural properties of OMS-2 during the self-assembly of mycelium pellet,both the crystal structure and the chemical structure were unchanged.In the catalytic degradation experiment of X-3B,OMS-2/hyphae showed a good catalytic effect,degrading 80% at 25 ? and natural p H for 35 minutes.After repeated use of OMS-2/mycelium for 4 times,X-3B was still degraded by 65%.Characterization of the OMS-2/mycelium after use showed that the OMS-2 on the surface of the mycelium did not fall off,and the structure of the material did not change after use,which can be effectively recycled..