Studies On The Anti-diabetic Activity Of Pseudolarix Acid B And The Mechanism Of Relieving Insulin Resistance

Diabetes mellitus is a metabolic syndrome characterized by chronic hyperglycemia caused by various factors such as heredity,obesity,immune dysfunction and environment.Type II diabetes mellitus is mainly caused by insufficient insulin secretion and insulin resistance.Insulin resistance is the main cause of type II diabetes mellitus,and it is also the main direction of research.Insulin resistance refers to the decrease in the sensitivity of insulin-effecting target organs to insulin action.The biological effects produced by normal doses of insulin are lower than those of normal biological effects.Therefore,insulin resistance makes the decline of the peripheral groups to take up glucose ability,and the raising of glucose bloods.Insulin production mainly includes hepatocytes,adipocytes,and skeletal muscle cells.In clinical practice,HepG2 cells are commonly used for insulin resistance studies caused of HepG2 cells are derived from hepatocyte strains,are similar to hepatocytes,while are easy to culture.Relieving insulin resistance is the focus of research on type 2 diabetes.Therefore,a new type of peroxisome proliferator receptor（PPARγ）and low toxic side effects drugs are urgently needed in clinical practice.Turtle skin is the dry root bark or near bark of the pine plant Pseudolarix kaempferi Gord.Its gas is mild,spicy and mild.It has insecticidal and antipruritic properties and is commonly used to treat snoring disease,Pseudolaric acid B（PAB）molecular formula is C₂₃H₂₈0₈.In this study,studies on the Anti-diabetic activity of Pseudolarix Acid B and the mechanism of relieving insulin resistance.1.Insulin resistance model of HepG2 cells was constructed with high concentration of insulin,Endoplasmic reticulum stress and mitochondrial damage model were induced by tunicamycin（Tun）and oligomycin（Oli）,respectively.Different concentrations of soil acetic acid（PAB）alleviated insulin resistance in glucose metabolism,and ELISA experiments confirmed that PAB promotes insulin release from insulin beta cells.2.Study on the Mechanism of Stagnation of Insulin Resistance by Hemoglobin Acetate in HepG2 Cells.Western-Blotting method was used to detect the protein expressions of PPARγ,IRS-1,Akt,P-akt,Grp78 and other proteins of HepG2 cell proliferation signal pathway when IR-HepG2 cells were treated with different concentrations of PAB.3.Study on Anti-diabetic Activity of Stilbene Acetic Acid.A mouse model of type 2 diabetes was constructed using high-sugar,high-fat,and STZ.Mice were intraperitoneally injected with different concentrations of PAB to measure the metabolic parameters of type 2 diabetes such as blood glucose,insulin,cholesterol,and triglycerides.In the paper,we have following the result the Anti-diabetic activity of Pseudolarix Acid B and the mechanism of relieving insulin resistance.1.The best model for insulin resistance is 10μM insulin-induced HepG2 cells for36 hours,PAB of 10μM can significantly relieve HepG2 insulin resistance,endoplasmic reticulum stress,mitochondrial damage,and increase glucose consumption.2.After the different concentrations of PAB acting on insulin-resistant HepG2cells for 24h,with the increase of PAB concentration,the expression of PPARγ,IRS-1and P-Akt was up-regulated,and the expression of GRP-78 was down-regulated.3.PAB can significantly reduce the blood glucose level,increase the insulin concentration,and improve the metabolic problems of diabetes such as triglyceride and total cholesterol in diabetic model mice.With the increase of PAB concentration,the significant increase in PAB can be achieved.Some indexes are stronger than the positive drug Rogeley ketone.The following conclusions were obtained through experiments:PAB can alleviate IR-HepG2 cells.PAB attenuates IR-HepG2 cells by influencing PPARγand IRS-1 to insulin receptors.PAB Anti-Type 2 Diabetes Activity by Relieving Insulin Resistance.The above studies provide new ideas for broad screening of anti-diabetic drugs.