Urine And Serum Metabolomics Study On Preeclampsia Patients

Urine and serum metabolomics study on preeclampsia patients based on Ultra high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC/QTOFMS)was used to analysis the changes caused by preeclampsia.In our study,30 cases of preeclampsia patients and 30 cases of normal pregnant women urine and serum were selected to screen the potential makers of preeclampsia.15 different metabolites were identified by the multivariate statistical analysis in urine,which were involved in tryptophan metabolism,glycolysis or gluconeogenesis,citrate cycle(TCA cycle).And 49 biomarkers were identified in serum.Amone those biomarkers,the PC(14:0/00),proline betaine and proline were potential sensitive and specific biomarkers for preeclampsia diagnosis and prognosis.Perturbation of corresponding biological pathways,such as i NOS signaling,nitric oxide signaling in the cardiovascular system,mitochondrial dysfunction were responsible for the pathogenesis of preeclampsia.We also followed up the difference of postpartum urine metabolism between normal group and preeclampsia group.We found that there was no significant difference between the prenatal and postpartum comparison in the normal group and the significant difference between the antenatal and postnatal in severe eclampsia group,which indicated that the changes in the early screening markers existed in the process of preeclampsia and had a certain correlation with the onset of preeclampsia.This study indicated that the metabolic profiling had a good clinical significance in the diagnosis of preeclampsia as well as in the study of its pathogenesis.It provided a clue for in-depth understanding the preeclampsia pathogenesis of patients with preeclampsia.It also suggested that liquid chromatography-mass spectrometry combined with multivariate analysis of urine metabolic study in obstetric disease early diagnosis and disease mechanism research showed great potential.