| ObjectivesTo detect serum metabolite levels in patients with preeclampsia and normal pregnant women before delivery,and to explore the correlation with cardiovascular function and the value of combined detection in the diagnosis of preeclampsia.Methods1.A total of 30 preeclampsia patients who were treated and delivered at Qingdao University Affiliated Hospital from December 2021 to May 2022 were selected as the observation group,including 20 mild preeclampsia patients and 10 severe preeclampsia patients.30 healthy pregnant women in the same period were selected as the control group.All pregnant women have signed informed consent forms.5ml of morning fasting venous blood of pregnant women in each group were collected and transferred into centrifuge tubes.Store blood samples in a-80 ℃ refrigerator for measurement.All selected participants underwent cardiac ultrasound and determination of N-terminal B-type natriuretic peptide precursor(NT pro BNP).2.Using liquid chromatography-tandem mass spectrometry(LC-MS/MS)metabolomics technology,the levels of taurine,glycine,L-carnitine,glutamate,and oleic acid in the serum of each group of participants before delivery were detected.Analyze the data using Analyst 6.3 mass spectrometry data processing software.3.Use SPSS26.0 statistical software to conduct statistical analysis on the data using one-way ANOVA,t-test,and Spearman correlation analysis.Analyze the diagnostic efficacy of five metabolic product levels in the serum of patients in each group on cardiovascular function in preeclampsia patients using the receiver operating characteristic curve(ROC)curve.ResultThere were no statistically significant differences in the levels of taurine,glycine,L-carnitine,glutamic acid,and oleic acid before delivery among different age,body mass index,fetal gender,gestational week,and parity groups(P>0.05).The observation group had lower levels of serum taurine and glycine compared to the control group,with statistically significant differences(P<0.05);The observation group showed high levels of L-carnitine,glutamate,and NT pro BNP compared to the control group,with statistically significant differences(P<0.05);There was no significant difference in serum oleic acid levels between the observation group and the control group(P>0.05).2.The left ventricular ejection fraction(LVEF)in the observation group was lower than that in the control group,and the left ventricular end systolic diameter(LVDs)and left ventricular posterior wall thickness(LVPW)were higher than those in the control group.The difference was statistically significant(P.The LVEF of patients with severe preeclampsia was lower than that of patients with mild preeclampsia,and the difference was statistically significant(P<0.05).There was no significant difference in other cardiac ultrasound indicators(P>0.05).3.The serum levels of L-carnitine,glutamate,and NT-pro BNP in severe preeclampsia patients were higher than those in mild preeclampsia group,with statistical significance(P<0.05).The serum levels of taurine and glycine in severe preeclampsia patients were not significantly different from those in mild preeclampsia patients(P>0.05).Spearman correlation analysis showed that serum taurine and glycine were negatively correlated with the severity of preeclampsia(r=-0.525,-0.427,P=0.000,0.002,respectively),while levels of L-carnitine and glutamate were positively correlated with the severity of preeclampsia(r=0.821,0.757,P=0.000,0.000,respectively).Comparing the diagnostic efficacy of independent and combined metabolites in the diagnosis of preeclampsia patients,the AUC of the combination of L-carnitine and glutamate in the diagnosis of preeclampsia patients was 0.983,the sensitivity was 96.7%,and the specificity was 100%.It has high predictive diagnostic efficacy and economic benefits.5.Serum levels of L-carnitine,glutamate,and NT-pro BNP were negatively correlated with LVEF(r=-0.642,-0.521,-0.434,P<0.05),while glycine was positively correlated with LVEF(r=0.340,P<0.05).Taurine was not significantly correlated with LVEF(P>0.05)Conclusion1.The levels of serum metabolites taurine,glycine,L-carnitine,and glutamate are related to preeclampsia,among which the levels of L-carnitine and glutamate have a certain evaluation effect on the severity of preeclampsia.2.Preeclampsia affects the morphology and function of the patient’s heart.As the condition of preeclampsia worsens,the decrease in the patient’s cardiac systolic function becomes more pronounced.3.The levels of L-carnitine,glutamic acid,and NT-pro BNP in serum are correlated with cardiac dysfunction in preeclampsia patients,and can be used to evaluate the cardiac function status of preeclampsia patients,thus providing early intervention and treatment.3.Serum L-carnitine,glutamic acid and NT-pro BNP levels are related to cardiac function decline in patients with preeclampsia,which can be used to evaluate the cardiac function status of patients with preeclampsia,so as to conduct intervention and treatment as early as possible. |
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