Studying The Mechanism Of AQP2,AQP3 And MicroRNA-155 To Blood Deficiency And Wind Dryness Type Atopic Dermatitis

Objective:Detecting the relative immune factors and aquaporins about atopic dermatitis(AD)to explore the TCM pattern of syndrome inner correlation among mi R-155,AQP2,AQP3 in patients dander and LDL,TG in serum,to further revealing the pathogenesis of blood deficiency and wind dryness atopic dermatitis,and provide an objective quantitative basis for the TCM syndrome differentiation of the disease.Methods: Taking the real time fluorescence quantitative PCR method to detect the relative expression quantity of mi R-155,AQP2 and AQP3 in injured skin and normal scurfy.Take U6 as an example,with the 2-Δ Δ CT method to count the mi R-155,AQP2,AQP3 relative expression quantity among atopic dermatitis(AD)patient.Using the fluorescence polarization immunoassay method to test LDL,TG in atopic dermatitis(AD)patient’s serum relative expression quantity and analysis it with SPSS21.0.Results :(1)The relative expression quantity of mi R-155,AQP3 in injured skin of patient with blood deficiency and wind dryness type are higher than those with spleen deficiency and dampness accumulation type and normal control group,the difference has statistically significant(P<0.05).The relative expression quantity of mi R-155,AQP3 in injured skin of patient with spleen deficiency and dampness accumulation type are higher than normal control blood,the difference has statistically significant(P<0.05).(2)There was no clear expression of AQP2 among three group with blood deficiency and wind dryness type,spleen deficiency and dampness accumulation type,normal control group.The relative expression quantity of LDL,TG in blood deficiency and wind dryness type patient serum are higher than spleen deficiency and dampness accumulation group and normal control group,the difference has statistically significant(P<0.05).The relative expression quantity of LDL,TG in spleen deficiency and dampness accumulation type are higher than normal control group,the difference has statistically significant(P<0.05).(3)The atopic dermatitis(AD)expression quantity of AQP3 were strongly correlated with mi R-155、TG,and had a weak correlation with LDL,the difference has statistically significant(P<0.01).The expression quantity of mi R-155 was weak correlation between TG and LDL,the difference has statistically significant(P<0.01).Conclusion:(1)The mi R-155,AQP3 existed in atopic dermatitis(AD)scurfy,it can be detected by real time fluorescence quantitative PCR method.Since there is no AQP2 expression in atopic dermatitis(AD)patient scurfy,it can be seen that may be no specific relationship of disease starting,developing of AQP2.(2)The expression quantity of mi R-155,AQP3,LDL,TG in blood deficiency and wind dryness type in atopic dermatitis(AD)patient are higher than spleen deficiency and dampness type and normal control group.The expression level of mi R-155,AQP3,LDL,TG in injured skin of spleen deficiency and dampness accumulation type in atopic dermatitis(AD)patient are higher than normal control group.This is mostly related to the mechanism of inflammation-Lipid oxidative stresssenility.(3)The correlation between mi R-155,AQP3,LDL and TG was different.It existed different level among mi R-155、AQP3、LDL、TG,so during the syndrome differentiation treatment,taking the primary judgment bymi R-155 、 AQP3 、 LDL 、 TGlevel are good for objective quantification by TCM detecting and further to instruct the disease distinguish and treatment.