Effect Of Kui Jie Ning Acupoint Application On IL-6,gp130,JAK2 And STAT3 In Ulcerative Colitis Rats With Yang Deficiency And Stasis

Objective: To observe the effect of Kuijie Ning Acupoint Application(溃 结宁 膏 穴 位 敷 贴,KJNAA)on interleukin 6(IL-6),Glycoprotein 130,Janus kinase 2(JAK2),signal transducer and activator of transcription 3(STAT3)of ulcerative colitis(UC)rats with spleen and kidney yang deficiency,and to explore the mechanism of the treatment of ulcerative colitis.Methods: 52 Sprague-Dawley(SD)rats were randomly divided into blank control group(blank group),group of ulcerative colitis rats with spleen and kidney yang deficiency(model group),KJNAA for the treatment group(application group)and sulfasalazine for the treatment group(SASP group).Except the blank group,Others were lavage with Senna and adenine combined with external intervention,and then to Skin sensitization with oxazole and enema with oxazole.The blank group was given equal dose of physiological saline for gavage and enema.Later,the application group was posted KJNAA on the acupoint:Zhongwan,Qihai,Zusanli,Tianshux.Group SASP was applied to the same acupoint with drug-free plaster and gavage of sulfasalazine solution.The blank group and the model group were treated with the same acupoint with drug-free plaster,and the normal saline was given to the stomach.It lasts for 28 days.After the treatment,the general diet,drinking water and defecation were observed in the rats.Evaluated the disease activity index(DAI)and Histopathological after HE staining.The expression of IL-6,gp130,JAK2 and STAT3 in colonic tissues of rats were detected by Western blot analysis.Result:(1)In the course of making mode,the body weight of the blank group increased steadily,and there was no obvious change.The rest of the three groups were somnolence.hypotrichosiscurled up,loose stool,foul,purulent stools,weight loss and other symptoms after second weeks.After the treatment,the symptoms of the model group continued to not be relieved,while the body weight of the compress group and the SASP group increased slowly,the stool turned to shape,and the mental activity was better.(2)Compared with the blank group,the disease activity index(DAI),colonic mucosal damage index(CMDI)and histological damage index(TDI)of the model group increased significantly,P<0.01,the difference was statistically significant.Compared with the model group,the scores of DAI,CMDI and TDI in the application group and the SASP group decreased significantly after treatment,P<0.01,the difference was statistically significant.But the difference between the application group and the SASP group was not obvious.(3)Compared with the blank group,the expression of IL-6,gp130,JAK2 and STAT3 in colon tissue of model group,application group and SASP group increased significantly,the difference was statistically significant(P<0.01).Compared with the model group,the expression level of IL-6,gp130,JAK2 and STAT3 in the application group and SASP group decreased,the difference was statistically significant(P<0.01),compared with SASP group,the difference was not statistically significant(P>0.05).Conclusion:(1)The application of KJNAA can improve symptoms and signs in ulcerative colitis rats with spleen and kidney yang deficiency.reduce the histopathological injury of colon tissue and relieve intestinal inflammatory reaction in rats.(2)The acupoint application of KJNAA can reduce the expression of IL-6,gp130,JAK2 and STAT3 protein in ulcerative colitis rats with spleen and kidney yang deficiency.