The Regulation Of Wnt/β-catenin Signaling On IL-21-mediated Macrophage Polarization

Background:Polarization of macrophages can play a role in promoting inflammation and resisting inflammation,respectively,and has important implications in immune regulation.IL-21 is a cytokine with important biological effects.It can bind to various receptors on immune cells,especially in macrophages.Interleukins can induce macrophage polarization,and the relationship between IL-21 and polarization has not been elucidated.Wnt signaling plays an important role in cell growth,differentiation,and apoptosis.It also plays an important role in immune regulation.It is important to investigate the relationship between Wnt/β-catenin signaling and macrophage polarization and IL-21.Objective:To explore the role of Wnt/β-catenin signaling in the regulation of IL-21-induced macrophage polarization.Methods:Immunoblotting was used to detect the effect of IL-21 on Wnt signaling.Flow cytometry and Western blotting were used to detect IL-21-stimulated macrophages after polarized surface markers,detection of Wnt3a and IL-21 treatment,and Wnt3a and IL respectively.-21 co-treatment of mouse macrophages 24 hours after the polarization-related protein expression and inflammatory factor expression.Result:1.After IL-21 stimulation of mouse macrophage RAW264.7,the expression of β-catenin and CyclinD1 protein was significantly increased,while the expression of GSK-3β protein was decreased.In IL-21 treated group,β-catenin Protein expression decreased,and GSK-3β protein expression increased slightly.When Wnt3a and IL-21 were co-treated,the expression of β-catenin and CyclinD1 protein was lower than that of Wnt3a treatment group.2.The expression of iNOS protein of M1 surface markers in IL-21 treated group was significantly lower than that in the control group.The expression of Arg-Ⅰ protein of M type surface marker was significantly increased compared with the control group.The flow-through results showed that the ratio of CD86 on the surface of M1 macrophages of the IL-21-treated group was significantly lower than that of the control group,and the ratio of CD206 on the surface of the M2-type macrophage was not significantly different from that of the control group.3.The expression level of Arg-1 protein in macrophages of Wnt3a and IL-21 co-treated group was lower than that of Wnt3a-treated group,and the level of SOCS3 protein was significantly higher than that of IL-21 group.The STAT3 protein level was significantly lower than that of the IL-21 group.Conclusion:Wnt3a may reduce IL-21-induced macrophage polarization,activation of Wnt/β-catenin signaling may inhibit IL-21-induced M2 polarization,and Wnt3a may attenuate IL-21-induced M2 Macrophage polarization induced cell repair.