The Correlation Of Radiotherapy Sensitivity And The Expression Of ANGPTL4 And STAT1 Proteins In Cervical Squamous Carcinoma

Objective: To research the correlation of radiosensitivity of cervical squamous carcinoma and expression of ANGPTL4 and STAT1 over human squamous cell carcinoma of the cervix.Methods: The expression level of ANGPTL4 and STAT1 proteins over 50 patients with squamous cell carcinoma of the cervix,30 patients with CIN and 20 patients with normal squamous epithelial tissue of the cervix was tested by immunohistochemistry.Analysis the expression of ANGPTL4 and STAT1 in different cervical tissue.According to the recent efficacy of radiation therapy,the patients were classified into high radiosensitive and low radiosensitive groups.The comparative analysis about the expression of those two proteins between different patients’ age,clinical stage and differentiation was implemented.Results:(1)The positive expression rates of ANGPTL4 in human squamous cell carcinoma of the cervix,CIN and normal squamous epithelial tissue of the cervix were 96%,90% and 15% separately,there was statistical significance among the three groups(P=0.000).The comparison results among three groups showed that there was no statistically significant difference between cervical cancer group and CIN group(P=0.574)while the difference over CIN and normal cervical tissue had statistical significance(P=0.000)so was in the normal cervical squamous epithelium and cervical carcinoma group(P=0.000).(2)The positive expression rates of STAT1 over human squamous cell carcinoma of the cervix,CIN and normal squamous epithelial tissue of the cervix were 88%,86.67% and 15% separately,there was statistical significance among the three groups(P=0.000).The comparison results among three groups showed that there was no statistically significant difference between cervical cancer group and CIN group(P=0.072)while the difference over CIN and normal cervical tissue had statistical significance(P=0.000)so was in the normal cervical squamous epithelium and cervical carcinoma group(P=0.000).(3)It had no statistically significant difference(P>0.05)among different cervical cancer patients’ ages,clinical stages and differentiations over the differences of ANGPTL4 and STAT1.(4)It has showed that there was correlation(r=0.645,P=0.000)over the expression of ANGPTL4 and STAT1 in cervical squamous carcinoma by Pearson correlation analysis.(5)The positive percentages of ANGPTL4 and STAT1 over high and low radiosensitive group were 90% and 85%、100% and 90% separately,there was no statistically significant differences between both of them(P>0.05).(6)The positive rates of ANGPTL4 and STAT1 in prognosis good group and prognosis bad group were 94.11% and 88.24%、100% and 87.5% separately,there was no statistically significant differences between both of them(P>0.05).Conclusion:(1)The expression of ANGPTL4 and STAT1 in cervical squamous carcinoma was higher than that in normal cervical squamous epithelium,it suggest that those two proteins may be involved in the development of cervical squamous carcinoma.But those two marks still canot make a definite diagnose on cervical squamous carcinoma.(2)There was no clear relationship between patients’ ages,differentiations and clinical stages over the expression of ANGPTL4 and STAT1.(3)ANGTPL4 and STAT1 may be distributed in the same molecular pathway which controls tumor progression,and also ANGPTL4 is located in the upstream of STAT1,the activation of ANGPTL4 could directly increase the expression level of STAT1.(4)It had no correlation with levels of ANGPTL4 and STAT1,over the radiosensitivity and prognosis.(5)The clinical stage can be used as an independent predictor in forecasting patients,radiosensitivity and prognosis,the high clinical stage lead to radioresistance,and poor prognosis.