The Inhibitory Effect Of Carboxymethyl Pachyman On HepG2 Cells And Its Apoptosis

Poria cocos is a traditional chinese herbal medicine,its main ingredient is poria polysaccharide,the chemical improvement of poria polysaccharide has a high anti-tumor activity.Hepatocellular carcinoma is the second disease and death rate of the disease,carboxymethyl pachyman polysaccharide has enhanced immunity,anti-tumor,anti-virus,anti-radiation,liver,hypnosis and other extensive pharmacological activity.In this paper,the aggregation of carboxymethyl pachyman in the solvent,combined with carboxymethyl pachyman polysaccharide on human hepatocellular carcinoma cell line HepG2 inhibition effect,screening better inhibition of tumor cells of carboxymethyl pachyman polysaccharide samples,through the To study the mechanism of apoptosis of HepG2 cell line induced by carboxymethylpachymycin polysaccharide in combination with endogenous signal pathway and exogenous signal pathway.The main results of this paper are as follows:1.The results showed that carboxymethyl pachyman could inhibit the proliferation of HepG2 cells,and the inhibitory rate of HepG2 cells was lower than that of other carboxymethylpachymates,and the inhibitory rate of HepG2 cells was lower than that of other carboxymethylpachymates sample.The inhibitory rates of the other six samples of the same concentration of carboxymethylpachymate were significantly different from those of CMP7 by SPSS 22.0 software（P<0.01）.Where CMP7 is an irregularly distributed,CMP1and CMP2 are clustered in the solvent and CMP3,CMP4,CMP5 and CMP6 are spherically distributed in the solvent.The results showed that the inhibition rate of HepG2 hepatocarcinoma cells was less than that of spherical or spherical spherical samples.2.The IC50_24h,IC50_48h and IC50_72h were 763.64±5.44μg/mL,34.06±1.09μg/mL and 26.34±0.77μg/mL,IC50_48h and IC50_72h,IC50_72h and IC50_72h,respectively.The inhibitory rate of CMP3 on HepG2 cells was analyzed by SPSS 22.0（P<0.01）,IC50_48h compared with IC50_72h,there was significant difference（P<0.05）.3.Flow cytometry was used to detect the apoptosis rate of HepG2 cells in HepG2cells.The apoptotic rate of HepG2 cells was 3.27%.The apoptosis rate of HepG2cells was 13.21%,and the apoptotic rate of HepG2 cells was 5-Fu 15.91%.Compared with HepG2 cells,the apoptotic rate of HepG2 cells and HepG2 cells was significantly higher than that of HepG2 cells（P<0.01）.The results showed that carboxymethyl Pachyman could induce HepG2 cell apoptosis.4.The results of western blot showed that the ratio of Bcl-2/Bax could decrease the expression of Bax,Caspase-3,p53 and cyto C,and down-regulate the expression of Bcl-2 and Bcl-2 Inhibit the expression of protein,and further induce apoptosis of HepG2 cells.5.The results of real-time quantitative PCR showed that CMP could effectively upregulate the mRNA expression of Fas,FasL and FADD in the death receptor pathway and promote the expression of Caspase-3,Caspase-8 and Caspase-Induced apoptosis of HepG2 cells.6.Carboxymethyl pachyman polysaccharide can induce HepG2 cell apoptosis through mitochondrial pathway,and induce apoptosis by death receptor pathway.