The Effect And Mechanism Of CNTF On Repair Of Cornea Nerves

ObjectiveTo explore the role of CNTF in the expression of p-STAT3 in the trigeminal ganglion of mice via in vitro normal mouse primary trigeminal ganglion cell culture model and Stattic-induced mouse epithelial injury repair model and to investigate the mechanism of CNTF in rat corneal nerve regeneration process.MethodsPrimary mouse trigeminal ganglion cells were cultured in vitro and divided into normal group,AGE group/Stattic group,AGE+ciliary nerve growth factor(CNTF)group/Stattic+ciliary nerve growth factor(CNTF)group.Western blot was used to detect the expression of p-STAT3 in ganglion cells.Immunofluorescence staining was to detect changes in axon growth of trigeminal ganglion cells.In vivo,streptozotocin(STZ)induces type I diabetic mice,and the expression of p-STAT3 in diabetic mice is detected by immunofluorescence staining and Western Blot.Normal mice were injected subconjunctivally with drugs and divided into normal control group,Stattic group and Stattic+CNTF group.The expression of p-STAT3 in trigeminal ganglion of mice was detected by Western Blot,and mouse epithelial wound healing model was established and sodium staining was used to observe the effects of CNTF on epithelial and corneal nerve repairResultsIn trigeminal ganglion cells cultured in vitro,compared with normal group,the expression of p-STAT3 in the AGE group was decreased,and the axonal growth of the trigeminal ganglion cells was significantly reduced(P<0.05);compared with the AGE group,the expression of p-STAT3 was increased in the ganglion cells after CNTF added,and the growth of axons was significantly increased(P<0.05).There was no significant difference between the normal group and the AGE+CNTF group(P<0.05).Compared with the normal group,the expression of p-STAT3 was decreased in the Stattic group,and the axonal growth of the trigeminal ganglion cells was significantly reduced(P<0.05).Compared with the Stattic group,the expression of p-STAT3 in the trigeminal ganglion cells was increased after addition of CNTF.The growth of axons was significantly increased(P<0.05);there was no significant difference between the normal group and the Stattic+CNTF group(P>0.05).In normal mice,in vivo,compared with the normal control group,subconjunctival injection of Stattic significantly decreased the expression of p-STAT3(P<0.05).There was no difference in the expression of p-STAT3 after Stattic dose increase(P>0.05).Compared with the normal control group,subconjunctival injection of Stattic in mice significantly inhibited corneal epithelial repair in mice(P<0.05);injection of CNTF after subconjunctival injection of Stattic,compared with Stattic group alone,corneal epithelial defect in mice was significantly reduced(P<0.05).ConclusionAGE reduces the expression of p-STAT3 in the trigeminal ganglion and inhibits the growth of axons in trigeminal ganglion cells.CNTF restored the expression of p-STAT3 in the trigeminal ganglion and promoted the inhibited axon regeneration of the trigeminal ganglion cells.The possible mechanism of CNTF on nerve regeneration may be through regulation of the STAT3 signaling pathway.