| Objectives: This study aims to investigate the relationships between the concentration of heavy metals and polycyclic aromatic hydrocarbons(PAHs)with the levels of oxidation damage to DNA and lipid in couriers from Guangzhou,and to lay a foundation to provide scientific basis data for the prevention and control of occupational diseases caused by heavy metal and polycyclic aromatic hydrocarbons.Methods: Huangpu District of Guangzhou was selected as a research area by a random sampling method,and 60 couriers in the area were selected as an exposure group(years of service ≥ 2 years),and 31 college students from the college town where was the low exposure area were surveyed as the low exposure group.The concentrations of nine heavy metals(cadmium,Cr;copper,Co;nickel,Ni;manganese,Mn;cooper,Cu;cadmium,Cd;zinc,Zn;arsenic,As;lead,Pb)in fine particulate matter of research area urinary were measured by inductively couple plasma-mass spectrometer.Concentrations of 10 OH-PAHs in the urinary of studied population were detected by UPLC-MS/MS.And the levels of 8-OHdG and 8-iso-PGF2α were determined by enzyme-linked immunoassay.Results: The internal exposure levels of heavy metals,in the urine of couriers were significantly higher than those in the control group(P<0.05).The principal component analysis results showed that the characteristic value of the main factor 1 in the exposed area was the largest,which explained 48.067% of the total variable.The loading values of Cr,Mn,Ni,Cu,and Zn were much higher than those of other heavy metals(0.942,0.945,0.807,0.639 respectively),indicating that there is a good correlation.In the multiple linear regression analysis model,the levels of 8-OHdG in the couriers increased with the increase of the urine As concentration,presenting a dose-effect relationship(β=0.565,P=0.003).However,with the increase of the content of Zn,the levels of 8-OHdG decreased,presenting a dose-effect relationship(β=-0.410,P=0.004).The levels of urine 8-iso-PGF2α increased with the increase of Cr,Cu and As concentration,and the dose-effect relationship was observed(β=0.054,P=0.009;β= 0.029,P = 0.060),and decreased with the increase of the concentration of Co,Ni and Zn,presenting a dose-effect relationship(β=0.158,P= 0.037;β=-0.159,P=0.073).The levels of 8-OHdG in couriers’ urine increased with the increase of 2-OHFlu and 2-OHPhe content,presenting a dose-effect relationship(β=0.296,P=0.041;β=0.106,P=0.028).The levels of urine 8-iso-PGF2α was increased with the increase of 2-OHPhe and 1-OHPyr concentration,presenting a dose-effect relationship(β=0.167,P=0.025;β=0.039,P=0.032).Conclusions: The levels of PAHs and heavy metals in couriers’ urine were higher than those of college students.The result of principal component analysis showed that the 9 sources of heavy metals in the exposed area were mainly from vehicles and industrial sources,followed by biomass combustion and coal combustion.The exposure of As in the complex traffic environment where couriers worked may cause oxidative damage and lipid peroxidation damage of DNA,and the exposure of Cr and Cu may cause lipid peroxidation damage,and Zn may be an important antioxidant factor in the antioxidant defense.The co-exposure of phenanthrene and fluorene of PAHs may cause the levels of oxidative damage of the couriers’ DNA to increase;the co-exposure of plutonium and phenanthrene may cause an increase in lipid peroxidation damage levels. |
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