Preliminary Studies On Mechanism Of Improving Sleep And Ethology Effect On Parkinson Drosophila By Kissoone B Of Valeriana Amurensis

Objective:to preliminary explore monomer compounds Kissoone B to improve the mechanism of action of sleep.Methods:1.Using sodium pentobarbital sleep experiment,together with the male mouse as the research object,which is based on mice is the reflection of the state,investigate Kissoone B synergy on threshold dose of pentobarbital sodium in mice sleep latency and sleep duration;Examine Kissoone B synergy subthreshold dose of sodium pentobarbital affect the incidence of sleep in mice2.The application of enzyme-linked immunosorbent kit,detection for Kissoone B in the prefrontal cortex,hippocampus and hypothalamus in mice after 5-HT,5-HIAA content and 5-HIAA/5-HT ratio.3.The application of enzyme-linked immunosorbent kit,detection for Kissoone B in the prefrontal cortex,hippocampus and hypothalamus in mice after the content of GLU4.Using enzyme-linked immunosorbent kit,detection for Kissoone B in the pref-rontal cortex,hippocampus and hypothalamus in mice af’ter DA,DOPAC and HVA content and DA/DOPAC,HVA/DA,the ratio of(DOPAC+HVA)/DA5.The cause of Parkinson’s with rotenone Drosophila models were given Kissoone B after climbing ability of Drosophila.Results:1.Compared with the blank control group,monomer compounds Kissoone B each dose group could shorten the incubation period of sleep in mice,and with a trend of decreasing drug dose escalation,but only for the high dose group was statistically significant(P<0.05).At the same time,Kissoone B each dose group and prolong the sleeping time of mice to extend the trend with increasing doses,among them,the low dose group and middle dose group(P<0.05),high dose group were markedly improved effect is significant(P<0.01).Monomer compounds Kissoone each dose group compared with the blank group B can extend the incidence of sleep in mice,and the increasing trend with drug dose escalation,among them,the dosage of dose group(P<0.05)and high dose group(P<0.01)there is statistical significance.2.Compared with the blank group,Kissoone dosage group B in the mouse brain hippocampus area 5-HT concentration increased significantly(P<0.05),the concentration of the hippocampus in mice brain area 5-HI A A increased significantly(P<0.05),while the prefrontal cortex and the 5-HT in the hypothalamus and 5-HIAA concentration has no obvious effect(P>0.05).Various brain regions in mice 5-HIAA/5-HT ratio had no significant influence3.B dosage group compared with blank group,Kissoone prefrontal cortex and the hypothalamus in mice brain area glutamate concentration significantly decreased(P<0.05),and glutamic acid concentration in hippocampus despite lower trend,but there was no significant difference4.B dosage group compared with blank group,Kissoone prefrontal cortex and hippocampus in mice brain area dopamine concentrations significantly decreased(P<0.05),and dopamine concentrations in the hypothalamus despite lower trend,but there was no significant difference.B dosage group compared with blank group,Kissoone prefrontal cortex in mice brain areas areas DOPAC levels(P<0.05)and DA/DOPAC ratio significantly higher(P<0.01),while in the hippocampus and hypothalamus DOPAC concentration and DA/DOPAC ratio had no significant difference.B dosage group compared with blank group,Kissoone in mice brain regions HVA and HVA/DA had no significant ef-fects;In the prefrontal cortex and hippocampus(DOPAC+HVA)/DA ratio has significant influence,in the hypothalamus(DOPAC+HVA)/DA ratio has no significant differenceConclusion:Kissoone B by influencing the 5-HT,DA,and the two related metabolic and GLU work together to improve sleep.5.Middle dose group and high dose group of male male flies climbing time decreased significantly after administration,climbing capacity significantly increased(p<0.01).High-dose group f’emale flies climbing time was reduced after dosing,statistically significant(p<0.05).The rest of the treatment group improved trend flies climbing ability,but bot statistically significant.