Fingerprint And Preliminary Pharmacokinetic Study Of Sulfated Hetero-polysaccharide(UF)Extacted From Saccharina Japonica

Sulfated polysaccharides extracted from brown algae have been paid much attention due to their diverse biological activities such as anti-oxidation,anti-tumor,anti-inflammatory and anticoagulaiton.There are many studies focus on fucoidan,but the sulfated hetero-polysaccharides are less concerned.In our early study of dozens of brown algae polysaccharides,a novel sulfated hetero-polysaccharides(UF)was shown to have significant neuroprotective effects.UF was measured to be a heteropolysaccharide with complex components and low sulfate content.Their quality control and pharmacokinetics is relatively difficult because of its complex composition and has become a cottleneck factor restricting drug development of UF.The fingerprint of UF was preliminarily established by HPLC.8 peaks were identified as D-mannose,D-Mannuronic acid,L-rhamnose,D-glucuronic acid,Dglucose,D-galactose,D-xylose and L-fucose.The similarity evaluation for chromatographic fingerprint indicated the method can be applied to the quality control of UF.Concerning the structural characteristics of UF,a fluorescent labelled polysaccharide FITC-UF was prepared by using isothiocyanate fluorescein(FITC)as label.The analysis results of UV –Vis and IR spectroscopy proved that FITC had been successfully connected to UF.The fluorescence intensity of FITC-UF was detected to be strong and stable.CCK-8 cytotoxicity test showed that FITC-UF sample had no cytotoxicity on PC12 cells and could be used in animal experiments subsequently.The results of methodology validation proved method to be accurate,rapid and sensitive and can be a foundation for detecting a large amout of biological samples in pharmacokinetic experiments.UF sample was detected in serum and organs of ICR mices after intraperitoneal administration.The pharmacokinetic datas showed UF was absorbed rapidly in the body and had obvious concentration accumulation in a short period of time.The concentration of UF entering body circulation was high but its metabolism rate was high too.The proportion of UF in organs was large.The detection of UF in brain illustrated that UF could be transported through blood-brain barrier and had a certain accumulation in brain.The establishment of the quality control method and the results of preliminary pharmacokinetics study for sulfated hetero-polysaccharide extracted from Saccharina japonica can provide a theoretical basis for solving the important problems in the process of UF drug development.These results also can speed up the development of UF as an innovative drug to a certain extent.