| Background: Hashimoto's Thyroiditis(HT)is an organ characterized autoimmune thyroid disease.The possible pathogenesis is related to genetic,immune and environmental factors.However,the specific molecular mechanism is not yet clear.Traditional research on the molecular mechanism of protein function research is generally based on existing work on a number of proteins for analysis,there may be subjective,one-sided and inefficient.With the renewal of technology,proteomics technology has become an important part of post-genome research.Differential proteomics can be used to compare disease-related differential proteins by comparing changes in protein expression levels between samples,providing favorable help for early diagnosis and reasonable treatment of various diseases.At present,proteomics for the study of thyroid diseases mainly focuses on the exploration of specific biomarkers of thyroid tumors,but few studies have reported that this method is used for the study of differential proteins in HT.Objective: The purpose of our study was to screen differential proteins in HT using isobaric tags for relative and absolute quantification(i TRAQ)proteomics,and analyze the possible mechanisms of these differential proteins in the development and progression of HT.Methods: we collected patients with HT(with or without nodules)and euthyroid controls at the Department of Endocrinology in the First Affiliated Hospital of Nanjing Medical University from November 2016 to April 2017.All specimens were from thyroid tissue nearby nodules using ultrasound-guided fine-needle aspiration biopsy.The proteins were extracted from these specimens and then the differential proteins were identified and analyzed by i TRAQ quantitative proteomics.Results: A total of 48 cases were collected,including 24 patients with HT(23 female and 1male,mean age 41.38 ± 12.99 years)and 24 euthyroid controls(19 female and 5male,mean age 48.54 ± 11.67 years),The i TRAQ quantitative proteomics was used to screen for differential proteins.Finally,A total of 911 differential proteins were identified.Furthermore i TRAQ quantitative proteomics revealed that 161 proteins were differentially expressed in experimental and control group according to statistical differences.Fifty five proteins selectively up-regulated and 106 proteins down-regulated in experimental group.Gene Ontology(GO)molecular function and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway revealed all up-regulated and down-regulated proteins participate in important biological functions,such as cellular process,single-organism process,metabolic process,single-organism cellular process,biological regulation.We analyzed the differential proteins involved in multiple pathways and the biological functions,we further screened out Heat Shock Protein 90?(HSP90?),Coronin-1A,Heterogeneous nuclear ribonucleoproteins A2/B1(Hn RNPA2/B1),Intestinal alkaline phosphatase(IAP),Tripartite motif9(TRIM9)and aquaporin1(AQP1)that had significant difference.Conclusion: Differential proteins were found in HT thyroid tissue compared to normal thyroid tissue using i TRAQ quantitative proteomics technology.Some of the differentially up-regulated proteins may be related to the promotion of abnormal activation of effector T cells or increased expression of inflammatory factors,resulting in the pathogenesis or aggravation of autoimmune inflammation;and some down-regulated expression of differential proteins may be attenuated by inhibition of inflammatory pathways.These differential proteins may play an important role in the occurrence and development of HT. |
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