Expression Of IL-36γ In Human Lung Adenocarcinoma Tissue And Its Clinical Significance

Objective:To investigate the expression of interleukin 36 gamma(IL-36y)in cancer and adjacent non-cancerous tissues from patients with lung adenocarcinoma.Methods:Both lung adenocarcinoma tissues and their adjacent non-cancerous tissues were collected from 57 patients,who underwent surgical resection from March 2009 to February 2015 in the Third Affiliated Hospital of Soochow University.All surgical specimens were pathological confirmed diagnosis of lung adenocarcinoma and none patient had chemotherapy or radiotherapy before operation.Lung adenocarcinoma diagnosis were confirmed by postoperative pathological diagnosis.Real-time quantitative PCR was performed to detect the mRNA expression of IL-36 gamma in the lung adenocarcinoma tissues and adjacent non-cancerous tissues.In order to further study the localization and protein expression of IL-36 gamma protein,75 cases of lung adenocarcinoma tissue microarray were enrolled from Shanghai core Biotechnology Co.,Ltd.,to analyze the correlation of IL-36 gamma expression with clinicopathological variables and overall survival.Results:The results of Real-time-PCR showed that the expression of IL-36 gamma in lung adenocarcinoma tissues was significantly higher than that in adjacent tissues.The immunohistochemical staining results showed that IL-36 gamma protein was mainly present in cytoplasm,a small number of localization was in the nucleus of cells.The positive expression rate of IL-36 gamma in lung adenocarcinoma was 92%(69/75),which was higher than that in adjacent tissues 77%(58/75),and the difference was statistically significant.Besides,the expression of IL-36 gamma was associated with lung pathologic stage,and gender,but was not associated with age,tumor size,lymph node metastasis and clinical stage.According to the Kaplan-Meier analysis and Log-Rank statistical test,there were trends in the expression level of IL-36 gamma protein and the survival time of patients,but there was no statistical significance.Conclusion:Increased expression of IL-36 gamma in tumor tissues of patients with lung adenocarcinoma.These results suggest that IL-36 gamma may play an important role in the development and targeted therapy of lung adenocarcinoma.It also provides a theoretical basis for the further study of the role and mechanism of IL-36 gamma mediated tumor immune responses.