Effect Of Corilagin On Apoptosis And Chemotherapy Resistance In U251 Glioblastoma Cells And U251 Stem-like Cells

Objective: The purpose of this study was to investigate the effects of Corilagin on apoptosis and ABCG2 in glioma U251 cells and U251 stem cells,and to determine its effect on migration of glioma U251 cells.Methods: U251 cells and U251 stem cells were cultured and passaged.Different concentrations(50-200 μg/ml)of Corilagin were used to intervene.The relevant parameters were detected at 24h-72 h.The morphological changes of cells were observed by fluorescence inverted microscope;Apoptosis was detected by flow cytometry technique;the expression of ABCG2 mRNA was detected by RT-PCR.The migration ability of U251 cells was detected by scratch test and Transwell assay.Results:1.The growth of U251 cells in the control group was good,and the cells adhered to the wall and covered the bottom of the flask.Its cell structure is complete and the cell body is polygonal.With the gradual increase of culture time,the number and density of cells gradually increase.In the Corilagin intervention group,the U251 cells became smaller,the density decreased,and cell debris increased.With the increase of drug intervention concentration,the change was more obvious.2.The U251 stem cells of the control group were spherical or oval in shape and in a good state.The U251 stem cells grew in suspension.Asthe incubation time prolonged,the number and density of cells gradually increased.The number and density of U251 stem cells decreased after the intervention of Corilagin.It was found that a large number of cell debris was suspended in the culture medium and the cell spheres were lysed.With the increase of the drug concentration,the changes after intervention were more obvious.3.At the same time point,with the increase of drug concentration,the apoptotic rate of two kinds of cells gradually increased,and when the drug concentration was 100μg/ml,the apoptotic rate tended to be stable.At the same intervention concentration,the apoptosis rate of both cells gradually increased with the prolonged drug intervention.At the same intervention time and intervention concentration,the apoptosis rate of U251 stem cells was higher than that of U251 cells(P<0.05).4.At the same time point,as the drug concentration increased,the mRNA expression of ABCG2 in both cells was gradually increased.At the same intervention concentration,the mRNA expression of ABCG2 in both cells increased with the prolonged drug intervention.At the same intervention time and intervention concentration,ABCG2 mRNA expression in U251 stem cells was higher than U251 cells(P<0.05).5.The scratch test results showed that compared with the control group,Corilagin intervention significantly reduced the migration of U251 cells at 24h-72h(P<0.05).Conclusion: Corilagin can induce the apoptosis of glioma cells and stem cells and promote the expression of drug resistance genes in glioma cells and stem cells,and the above effects are more pronounced in glioma stem cells.At the same time,Corilagin can effectively inhibit the migration of glioma cells.