Effects Of Corilagin And Temozolomide On Proliferation And Apoptosis Of U373 Glioma Cells

Objective: To investigate the effects of Corilagin and Temozolomide on morphological changes,cell migration,cell proliferation,cell cycle and apoptosis of glioma cell line U373 and their effects on TNF-?,NF-?B and Caspase-3/7/9 mRNA expression.Methods: Cultured U373 cells were treated with different concentrations of Corilagin(25,50,100,200 ?g/ml)and Temozolomide(40,80,160,320 ?g/ml).At 24,48 and 72 h,the morphological changes of U373 cells were observed by optical microscopy,the proliferation activity of U373 cells was detected by Cell Counting Kit-8 kit,and the migration ability of U373 cells was detected by cell scratch assay.After 48 hours of drug intervention,the apoptosis and cycle of U373 cells were detected by flow cytometry,and the expression levels of NF-?B,TNF-? and Caspase-3/7/9 mRNA in U373 cells was detected by RT-PCR.Results: 1.U373 cells that have not been interfered with by Corilagin and Temozolomide have a good growth state,the structure is complete,and the cell body is round.With the prolongation of culture time,the growth density and volume of U373 cells are significantly increased.But,after Corilagin and Temozolomide intervene in U373 cells,The cell bodies have different degrees of shrinkage,and a large number of scattered cell debris can be seen.With the increase of drug concentration and the prolonged intervention time,the cell growth density and volume are also significantly reduced,and cell growth is significantly inhibited.2.The survival rate of U373 cells gradually decreased with the increase of drug concentration of Corilagin or Temozolomide under the same intervention time(P<0.05);Under the premise of the same drug concentration,the survival rate of U373 cells is also decreasing with the prolonged intervention time of Corilagin or Temozolomide(P<0.05).The modified kou's method used to calculate the half-inhibitory concentration of U373 cells after 24,48 and 72 h of Corilagin intervention was significantly lower than temozolomide.3.Compared with U373 cells without drug intervention,the migration of U373 cells treated with corilagin or temozolomide was significantly reduced.And as the concentration of the two drugs increases,the cell migration decreases more obviously(P<0.05).4.After 48 hours of intervention with Corilagin or Temozolomide,the apoptotic rate of U373 cells gradually increased with the increase of the concentration of the two drugs(P<0.05).Under the premise of increasing the concentration of the two drugs,the cell cycle was stagnant in the G2 M phase after the intervention of Corilagin;and after Temozolomide intervention,the cell cycle was arrested in the S phase.5.U373 cells were treated with Corilagin or Temozolomide for 48 h,with the concentration of the two drugs increased,the mRNA expression levels of TNF-? and NF-?B were generally down-regulated(P<0.05);and the mRNA expression of Caspase-3/7/9 is generally up-regulated(P<0.05).Conclusion: 1.Temozolomide can inhibit the growth of glioma U373 cells,induce cell apoptosis,inhibit cell migration,and arrest the U373 cell cycle in S phase.The mechanism may be related to down-regulation of TNF-? and NF-?B mRNA expression levels.2.Compared with temozolomide,the lower drug concentration of Corilagin can also effectively induce apoptosis of U373 cells,inhibit cell migration,and arrest the cell cycle in the G2 M phase.Therefore,Corilagin has a stronger inhibitory effect on the proliferation of glioma U373 cells,and its mechanism may be related to down-regulation of TNF-?,NF-?B,and up-regulation of Caspase-3/7/9 mRNA expression levels.