Effect Of Human Hepatic Stellate Cells On ΓΔt Cells And Its Effect On Hepatocellular Carcinoma

Objective:To investigate the effect of human hepatic stellate cells on the cytotoxicity of γδ T cells from healthy volunteers and hepatocellular carcinoma patients,and the effect of γδ T cells on the proliferation,invasion and migration of hepatocellular carcinoma.Methods: Density gradient centrifugation and flow cytometry were used to obtain γδ T cells;phytohemagglutinin and interleukin-2 were used to stimulate the proliferation of γδ T cells;human hepatic stellate cells and γδ T cells were co-cultured with transwell chamber;ELISA was used to detect the changes of cytokine INF-γ;CCK-8 assay was used to detect the proliferation of HepG2 cells;Transwell chamber assay was used to detect the invasion and migration ability of HepG2 cells.Results: After co-culture with HSC,the cytotoxicity of γδ T cells from healthy volunteers and patients with HCC was enhanced,and the former is stronger than the latter(P<0.05);IFN-γ secreted by γδ T cells was increased,and healthy volunteers group secreted more than those with HCC(P<0.05);The ability of γδ T cells to inhibited the proliferation,invasion and migration of HepG2 cells was enhanced,and the healthy volunteers were stronger than those of the patients with HCC(P<0.05).Conclusion: HSC can promote γδ T cells’ secretion of IFN-γ,enhance the cytotoxicity of γδ T cells and the ability of γδ T cells to inhibit the proliferation,invasion and migration of HCC,and the enhancement effect is related to the source of γδ T cells.