| Objective: To investigate whether E2 can cause the migration of papillary thyroid cancer K-1,BCPAP cells,To investigate the molecular mechanism of the stumination of p S2/TFF1(phosphate starvation-induced genetrefoil/factor family TFF)by 17β-estradiol(E2)in papillary thyroid cancer K-1,BCPAP cells.Methods: The level of pS2/TFF1 mRNA in K-1 and BCPAP cells treated by E2 was examined using the p S2/TFF1-ELISA Kit.The pS2/TFF1 protein level in supernatants was detected with p S2/TFF1-ELISA Kit after treatment with E2,PPT and DPN.ERα-si RNA and ERβ-siRNA was designed,the transfection efficiency of ERα and ERβ was detected by western blot.the supernatants of cells was tested by pS2/TFF1-ELISA Kit.chromatin immunoprecipitation analysis(CoIP)was used to examine the interaction of pS2/TFF1 promoter with ER.The migration of K-1 and BCPAP cells was tested by MTT after transwell.Results: After 24 hours’ treatment of E2,The mRNA levelof pS2/TFF1 reached a max value.The level of pS2/TFF1 in supernatants of cells were both increased after treated with E2 and PPT compared with control group.wheras,the level of pS2/TFF1 of which treated with DPN was decreased compared with control group.The protein level of pS2/TFF1 in cells that transfected with ERα-si RNA was decreased.Nevertheless,the level in those transfected with ERβ-siRNA was increased gradually.The result of CoIP indicated that ERα and c-jun protein interacted with eachother and then bind to the promoter of pS2/TFF1 gene in K-1and BCPAP cells.When treated with 10 nM E2 for 24 h,the OD570 for E2,PPT and silencing group of ERα,c-jun and c-fos in K1 and BCPAP cells were decreased compared with control group,rather DPN and ERβ-siRNA in K1 and BCPAP cells were incresed.Conclusion: E2 can promote the expression and secretion of p S2/TFF1.ERα can bound to c-jun to form a complex and then combine with AP-1 site.Actually E2 can also promote the migration of of K-1 and BCPAP cells. |
PDF Full Text Request