The Change Of The Ovarian Function In Female Rats With Intrauterine Growth Retardation

Objectives: The aim of this study is to find out the impact and the mechanism of the ovarian function in female rats with intrauterine growth retardation,so we can provide some theory basis for the therapy or fertility advise for the women who suffered from IUGR.Methods: We limit the nutrition of the pregnant rats and build the IUGR model of the offspring.When the offspring grow up to 28 days,we measured the serum testosterone hormones and serum AMH,the follicles of different stages and we used the TUNEL technology to detect the atersia follicles.In the second stage of this study,the production of the AMHR-II of the ovary was examined using immunohistochemistry and the production of the IGF-1 was examined using PCR and western blot experiment.SPSS13.0 software was employed to analyze the data and P<0.05 indicates singnificant difference.Results :(1)In the IUGR group,we found out that the percentage of the growing follicles increased(34.8% vs 31.1%)and the percentage of the primordial follicles decreased(65.2% vs 31.1%)compared with theNC group;(2)We observed the number of the atresia follicles were increased in the IUGR group;(3)Compared with the NC group,the serum AMH and testosterone hormones of the IUGR rats were increased significantly;(4)The production of the AMHR-II and IGF-1 in the IUGR group were increased compared with the NC rats.Conclusions:This study indicated that the nutrition restriction during gestation has influenced the follicle development and the function of the ovary,and it changed the micro-environment of the ovary,accelerating the recruitment of the primordial follicles,and may influence the fertility in the further.We should pay attention to it and make early therapy and intervention for the women who had suffered from IUGR.