Clinical Study On T And B Lymphocyte Subsets And NK Cells In Peripheral Blood Of Patients With Parkinson’s Disease

Objective: To study the levels of T and B lymphocyte subsets and NK cells in peripheral blood of patients with Parkinson’s disease,and to analyze whether the above subsets are correlated with the severity of clinical exercise symptoms.Methods: 105 patients with primary Parkinson’s disease were included as the case group and 103 healthy controls.The percentages of T and B lymphocyte subsets and NK cells in peripheral blood were detected by flow cytometry.Part III of the unified Parkinson’s disease scoring scale(UPDRS III)was performed in the case group and hoehn-yahr staging was used to evaluate the severity of the disease.Differences in the levels of appellate lymphocyte subsets between the case group and the control group were identified,and their correlation with disease severity was further explored.Results: The positive rate of CD3+T lymphocytes in peripheral blood of the case group was lower than that of the control group,and the difference was statistically significant(t=8.262,P<0.01).The positive rate of CD4+T lymphocyte subgroup in peripheral blood of the case group was lower than that of the control group,and the difference was statistically significant(t=12.107,P<0.01).The positive rate of CD25+T lymphocytes in peripheral blood of the case group was lower than that of the control group,and the difference was statistically significant(t=24.931,P<0.01).There was no significant difference in the positive rate of CD8+T lymphocyte subgroup between the two groups(t=1.266,P=0.207).There was no statistically significant difference in the positive rate of CD19+B lymphocytes(t=0.847,P=0.398),nor in the positive rate of CD56+NK cells(t=1.310,P=0.192).In stratified analysis,the positive rates of CD3+T,CD3+CD4+T and CD4+CD25+T lymphocyte subsets in the peripheral blood of male patients in the case group were lower than those in the control group,with statistically significant differences(P < 0.05).In the correlation analysis,CD3+T,CD3+CD4+T,CD4+CD25+T lymphocyte subsets in the case group were negatively correlated with UPDRS III score and hoehn-yahr staging(P<0.01).Conclusion: PD patients peripheral blood lymphocyte imbalance,T lymphocytes,helper T lymphocyte subgroup and regulatory T lymphocyte subgroup is relatively low level of healthy controls,and the heavier motor symptoms,its decline about obviously,tip cell immune may play a role in the pathogenesis of PD,can through the immune adjustment in the future to intervene in the onset and progression of PD.