| Objective:To study the distribution of the SNP polymorphisms of ELOVL2 and GCKR genes in a population of Anhui province,and to investigate the nutritional intake of dietary and PUFA in the population,and to analyze the correlation between the three SNP polymorphisms and the risk of coronary heart disease in a population of Anhui province.Methods:In this study,230 patients with coronary heart disease in the Department of Cardiology of Huainan First People’s Hospital were selected by case-control design.According to the principle of frequency matching,236 healthy controls matched by age,sex and nationality were collected in the physical examination center of Huainan First People’s Hospital.A questionnaire survey was conducted on the nutritional intake of dietary and PUFA among all staff.ELOVL2 gene rs 1570069 loci,rs2236212 loci and GCKR gene rs780094 loci were selected for genotyping by PCR-RFLP detection and Sanger sequencing analysis.The general situation(age,sex),genotype,allele frequency and genotype distribution in CAD group and healthy control group were analyzed by Excel 2010 and SPSS20.0 software.EpiData 3.1 software was used for data analysis.Questionnaire data were input and then analyzed with SPSS20.0 statistical software.Results:The average age of CAD patients was 58.08±14.57 years old,including 117 males(50.9%)and 113 females(49.1%).The average age of healthy controls was 56.14±9.22 years,including 116 males(49.2%).),120 female patients(50.8%).There were no significant differences between the CAD group and the control group in gender(X2=0.137,P=0.711)and age(x2=1.648,P=0.086).Different genetic models showed that:There were not statistically different in dominant model(x2=0.015,P=0.502),recessive model(x2=0.958,P=0.624),homozygous model(X2=0.931,P=0.335),super-dominant model(X2=0.142,P=0.707)with ELOVL2 gene rs1570069 polymorphism in CAD group and control group;There were no significant differences in the dominant model(x2=0.005,P=0.903)and homozygous model(X2=1.274,P=0.259),but there were statistical differences in the recessive model(X2=13.617,P=0.000,OR[95%Cl]=2.012[1.385-2.923])and the super-dominant model(X2=13.673,P=0.000,OR[95%CI]=2.000[1.381-2.890])with ELOVL2 gene rs2236212 polymorphism in CAD group and control group;There were no significant differences in the dominant model(X2=0.420,P=0.517)and homozygous model(X2=1.047,P=0.306),but there were statistical differences in the recessive model(X2=4.795,P=0.029,OR[95%CI]=1.678[1.053-2.801])and the super-dominant model(X2=5.344,P=0.021,OR[95%CI]=1.548[1.068-2.247])with GCKR gene rs780094 polymorphism in CAD group and control group;There was a statistical difference between the CAD group and the control group in the case of edible oil(P<0.05);There was a statistically significant difference between the CAD group and the control group in the frequent consumption of PUFAs-rich foods and foods(P<0.05).The CAD group and the control group were statistically different in the proportion of irritability and mood swings(P<0.05).Conclusion:There was no correlation between ELOVL2 rs1570069 polymorphism and coronary heart disease,The polymorphism of ELOVL2 rs2236212 locus is correlated with coronary heart disease,The polymorphism of GCKR rs780094 locus is correlated with coronary heart disease;The type of edible oil,irritable,and mood swings are risk factors for coronary heart disease.Frequent use of foods rich in PUFAs may reduce the risk of coronary heart disease.Figure[6]table[18]reference[54]... |
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