| Objective:Jaw defects are a major problem in the clinic today,and the emergence of guided bone regeneration(GBR)technology has brought new methods and hopes to solve this problem.It uses the barrier function of the membrane to regenerate bone tissue,combining some biological materials.In the previous study of the experimental group,a biological barrier membrane was prepared.The membrane was poly(propylene carbonate(PPC)-loaded with nano-hydroxyapatite(nHAp).The purpose of this experiment is to explore the ability of this biofilm in guiding the regeneration of bone defects and its biocompatibility in animals.The clinically more commonly used Heal-ALL membrane is a positive control.Method:1.Six New Zealand white rabbits were randomly divided into two groups,three in each group.Before the surgery,the rabbits were observed for one week.Anesthesia was injected intramuscularly according to the weight of each rabbit.After the anesthesia was effective,the iodophor disinfected area,the skin was prepared in the operation area,and the soft tissue was cut.Each rabbit parietal was made of four 6mm diameter circular bone defects that did not damage the meninges by the same bone drill,and each defect was separated by more than 2mm.One of the bone defect surfaces did not cover the biofilm as a blank control group,the other three bone defect surfaces were covered with nHAp/PPC membrane containing 10%by weight of nHAp,nHAp/PPC membrane containing 20%by weight of nHAp,and Heal-ALL biofilm.The biofilm was fixed with titanium nails to prevent movement.After the titanium nail was fixed to the biofilm,the skill is sutured.Postoperative rabbits were housed in a uniform environment.On the basis of the weight of the rabbit,intramuscular injection of penicillin daily to prevent infection.Two groups of rabbits were sacrificed at 4 and 12 weeks after surgery,and the rabbit paietal was removed and placed in a fixative for use.2.Radiological detection of the fixed bone tissue by Micro-CT,and three-dimensional reconstruction of the bone defect area using the relevant software to evaluate the new bone mass.The quality of the new bone was evaluated by data analysis of the number of trabecular bone,trabecular thickness and trabecular spacing of the new bone.3.Bone tissue was routinely decalcified,embedded and sectioned.And the quality of new bone was assessed histologically by HE staining and Masson staining.Results:No postoperative death occurred in the rabbit,and the operation area recovered well without abnormal inflammatory reaction.Micro-CT three-dimensional reconstruction images and bone tissue data analysis results showed that at 4 and 12 weeks,the experimental composition of the biofilms in the three groups was significantly higher than that of the biofilms in the absence of bone powder.In the group,20%of the nHAp/PPC group showed more significant bone regeneration.The histological analysis found that the biofilms of the three experimental groups played a role in guiding bone regeneration during the process of guiding bone regeneration to prevent soft tissue growth and provide the necessary space for bone tissue formation.Conclusion:In conclusion,the nHAp/PPC membrane showed good biocompatibility during rabbit skull defect repair,and the 20%nHAp/PPC membrane was more prominent. |
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