| Objective: To study the effect of Xuelian Injection on the balance of Th1/Th2 cells and the differentiation of Th17 cells in collagen-induced arthritis rats,and to explore its mechanism of action on rheumatoid arthritis.Methods: Thirty female Wistar rats were modeled.The rats were immunized with multi-point intradermal injection of bovine type II collagen emulsion(0.3 ml/mouse)at the ankle and tail of the rats.After successful modeling,they were randomly divided into collagen-induced arthritis(CIA)model group,Xuelian Injection group and Tripterygium group.After 28 days of treatment,the rats were sacrificed.The inflammatory changes were observed by pathological section.The expression of IL-2,IL-4,IL-17,IL-6 and IL-21 in synovial membrane was detected by immunohistochemical staining.The serum levels of IL-2,IL-4,IL-17,IL-6 and IL-21 were detected by the Enzyme-linked immunosorbent assay(ELISA)method.Results:Xuelian Injection significantly improved the severity of CIA rats.Compared with the model group,the joint synovial inflammation was improved in the Xuelian Injection group and the Tripterygium group after treatment.The expression levels of IL-2,IL-6 and IL-21 in the synovium was significantly decreased(P<0.05),the expression levels of IL-17 and IL-4 were not significantly changed(P>0.05),and the levels of IL-2,IL-6 and IL-21 in serum were significantly decreased.(P<0.05),there was no significant change in the expression levels of IL-17 and IL-4(P>0.05).Conclusion: According to the above results,Xuelian Injection may play the important role in the treatment of rheumatoid arthritis by regulating immune imbalance caused by Th1/Th2 cell imbalance and Th17 cell differentiation. |
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