| Objective: To detect the expression of human epidermal growth factor receptor 2(erbB2,HER2)and epidermal growth factor receptor 1(erbB1,EGFR)in primary and ovarian metastases of patients with ovarian metastasis,and to explore the biological significance of the expression of HER2 and EGFR in ovarian metastasis of colorectal cancer.Methods: From June 2013 to December 2017,31 patients with colorectal cancer with ovarian metastasis diagnosed and treated by gastrointestinal surgery of Cancer Medical College of Guangxi Medical University were collected,including 20 patients with matched specimens of primary and ovarian metastases,9 patients with ovarian metastases only received specimens of primary resection in the hospital.Metastases were operated on in the hospital,and only 2 cases of primary lesion specimens were obtained.At the same time,20 primary specimens of female colorectal cancer patients without ovarianmetastasis treated in our hospital during the same period were collected(including 3 cases with liver metastasis,1 case with bladder metastasis,1 case with lung metastasis,1 case with extensive abdominal metastasis,14 cases with mesenteric fat metastasis).The expression of HER2 and EGFR in primary and ovarian metastases of colorectal cancer patients with ovarian metastasis and those without ovarian metastasis were detected by immunohistochemistry.Collect and record the relevant clinical data of patients(including age,location of tumors(colon,rectum),maximum diameter of tumors,time of ovarian metastasis(simultaneity,asynchrony),peritoneal metastasis,degree of differentiation(high differentiation,medium-low differentiation),lymph node metastasis,intravascular tumor thrombus);analyze the expression of HER2 and EGFR in three groups of specimens concurrently.The correlation between the expression of HER2 and EGFR and the clinical status of patients was analyzed.The experimental data were analyzed by SPSS 22.0.Wilcoxon rank-sum test was used for rank data,and the measurement data were expressed by mean(+standard deviation)(?X±s).2 test and Fisher’s exact probability method were used to compare the differences between the counting data,t test was used to compare the differences between the measurement data(bilateral);Spearman correlation coefficient was used to analyze the correlation between the two variables;P < 0.05 was the difference between the two variables.Results: 1.The positive rate of HER2 was 54.5%(12/22)in primary tumors of patients with ovarian metastasis,44.8%(13/29)in ovarian metastasis and30.0%(6/20)in primary tumors of patients without ovarian metastasis.There was a significant difference in the expression of HER2 between primary tumors of patients with ovarian metastasis and those without ovarian metastasis(P<0.05).There was no significant difference in the expression of HER2 between primary and ovarian metastases in patients with ovarian metastases(P>0.05).There was significant difference in the expression of HER2 between ovarian metastasis and primary ovarian metastasis in patients with ovarian metastasis and non-ovarian metastasis(P<0.05).There was no significant correlation between the expression of HER2 in primary lesions of colorectal cancer patients with ovarian metastasis and the clinical factors such as age,location of tumors,differentiation degree,lymph node metastasis,maximum diameter of tumors and intravascular tumor thrombus(P>0.05).There was no significant correlation between the expression of HER2 in primary lesions of colorectal cancer patients without ovarian metastasis and clinical factors such as age,location,differentiation degree,lymph node metastasis,maximum diameter of tumors and intravascular tumor thrombus(P>0.05).HER2 in ovarian metastasis was correlated with peritoneal metastasis(P<0.05).HER2 in ovarian metastasis was not correlated with age,ovarian metastasis time(simultaneity,dyschronism),tumor location and other clinical factors(P>0.05).2.The positive rate of EGFR was 63.6%(14/22)in primary tumors of colorectal cancer with ovarian metastasis,69.0%(20/29)in ovarian metastasis and 65.0%(13/20)in primary tumors of colorectal cancer without ovarian metastasis.There was no significant difference in the expression of EGFR between primary tumors in patients with ovarian metastasis and those without ovarian metastasis(P > 0.05).There was no significant difference in the expression of EGFR between primary and ovarian metastases of colorectal cancer patients with ovarian metastasis(P>0.05).There was no significant difference in the expression of EGFR between ovarian metastasis and primary ovarian metastasis in patients with ovarian metastasis and colorectal cancer without ovarian metastasis(P>0.05).There was no significant correlationbetween the expression of EGFR in primary tumors of colorectal cancer patients with ovarian metastasis and clinical factors such as age,location of tumors,degree of differentiation,lymph node metastasis,maximum diameter of tumors and intravascular tumor thrombus(P>0.05).The expression of EGFR in non-ovarian metastasis primary lesions of colorectal cancer was not significantly correlated with clinical factors such as age,location,differentiation,lymph node metastasis,maximum diameter of tumors and intravascular tumor thrombus(P>0.05).The expression of EGFR in ovarian metastasis of colorectal cancer patients with ovarian metastasis was not significantly correlated with age,time of ovarian metastasis(simultaneity,asynchrony),peritoneal metastasis and tumor location(P>0.05).Conclusion: 1.HER2 and EGFR were highly expressed in primary and ovarian metastases of colorectal cancer patients.2.Whether the occurrence of ovarian metastasis of colorectal cancer is related to the high expression of HER2 in primary colorectal cancer should be further explored and studied.3.The high expression of HER2 in primary and metastatic lesions of colorectal cancer patients with ovarian metastasis suggests that the cells in primary and metastatic lesions of colorectal cancer may have some similarities in biological characteristics.Whether the cells with high expression of HER2 in primary lesions of colorectal cancer are more prone to ovarian metastasis deserves further study.4.The biological significance of high expression of EGFR in ovarian metastasis of colorectal cancer needs further verification. |
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