Correlation Between Serum Non-enzymatic Antioxidant Melatonin,Glutathione And Oxidative Stress In Parkinson’s Disease

ObjectiveTo investigate the changes of serum non-enzymatic antioxidant melatonin(MLT)and glutathione(GSH)in patients with Parkinson’s disease(PD),and to explore whether the trends of the two indicators are the same.What is the connection between the two?Is there any difference in the role of its antioxidant effect?To analyze their possible modes of action in Parkinson’s disease and to explore their relationship with the severity of Parkinson’s disease,and provide further reference for the mechanism of oxidative stress in Parkinson’s disease.In addition,study the relationship between melatonin and sleep in patients with Parkinson’s disease,and the relationship between glutathione and cognitive function in patients with Parkinson’s disease,provide new ideas for the treatment of these two non-motor symptoms in PD patients.Methods50 patients with Parkinson’s disease admitted to the Second Affiliated Hospital of Zhengzhou University from September 2017 to February 2018 were enrolled in the study.50 healthy controls from the physical examination center of the hospital were selected.The severity of Parkinson’s disease was assessed using the HY(corrected)rating scale.The cognitive function of the patients was assessed using the Montreal Cognitive Assessment Scale(Beijing Edition).Pittsburgh Sleep Quality Index was used for patients and controls.The sleep status of the population is assessed.Collecting venous blood from two groups of members from 7:00-9:00 in the morning.Serum melatonin(MLT)and glutathione(GSH)levels in PD patients and healthy controls were detected by enzyme-linked immunosorbent assay(ELISA),and the results were compared and analyzed.Results1.The serum MLT level in the PD group was significantly higher than that in the healthy control group [(84.12±6.58)pg/ml vs(46.29±9.73)pg/ml,P=0.000].Serum GSH levels in the PD group were significantly lower than those in the healthy control group [(21.07±12.05)μmol/L vs(77.73±39.90)μmol/L,P=0.000].2.The levels of serum MLT and GSH in the PD group were negatively correlated(r=-0.842,P=0.000).3.There was a positive correlation between serum MLT level and H-Y grade in PD group(r=0.537,P=0.000).There was a negative correlation between serum GSH level and H-Y grade in PD group(r=-0.596,P=0.000).4.The MLT level of PD group with sleep-disordered was significantly higher than that of the group without sleep disorder [(85.79±6.45)pg/ml vs(78.84±3.54)pg/ml,P=0.001].In the normal control group,the MLT level in the sleep disorder group was(49.33±9.75)pg/ml,and the MLT level in the non-sleep disorder group was(45.22±9.62)pg/ml.The difference was not statistically significant(P = 0.193).5.GSH levels in PD patients with cognitive dysfunction were significantly lower than those without cognitive dysfunction [(17.47±10.67)μmol/L vs(26.09±12.23)μmol/L,P=0.011].6.The serum MLT concentration in the PD group aged 40-60 years was(85.05±6.89)pg/ml;the serum MLT concentration in the 61-80 years old group was(84.43±6.36)pg/ml.The serum MLT concentration in the age group >80 years was(79.40±4.52)pg/ml.There was no significant difference in the age of PD patients(F=1.872,P>0.05).The serum MLT concentration in the control group aged 40-60 years was(48.36±9.80)pg/ml;the serum MLT concentration in the 61-80 years old group was(45.43±9.76)pg/ml;The serum MLT concentration in the age group >80 years was(40.87±8.04)pg/ml,and there was no significant difference between the age groups(F=1.591,P>0.05).7.The serum MLT concentration in the male PD group was(82.56±6.19)pg/ml.The serum MLT concentration in the female PD group was(85.96±6.67)pg/ml.There was no difference between the two groups.(t =-1.866,P = 0.068).The serum MLT concentration in the male control group was(47.74±9.17)pg/ml.The serum MLT concentration in the female control group was(44.58±10.28)pg/ml.There was no significant difference between the two groups.(t=1.149,P=0.256).Conclusion1.MLT and GSH may be involved in the pathogenesis of Parkinson’s disease.In the course of Parkinson’s disease,there may be some differences in the trends of the two and the way they act in the disease.2.MLT and GSH may be related to the severity of Parkinson’s disease,and they can be used as reference indicators for judging the severity of the disease.3.MLT may be associated with sleep disorders in patients with Parkinson’s disease,and GSH may be associated with cognitive impairment in patients with Parkinson’s disease.It provides a new direction of thinking for the treatment of two non-motor symptoms such as sleep disorders and cognitive disorders.