LncRNA LINC00936 Participates In The Development Of Ovarian Cancer By Adsorbing MiR-221-3p To Regulate The Expression Of LAMA3 Abstract

Objective:Ovarian cancer is one of the common malignant tumors in gynecology.The mortality rate is high,the incidence is concealed,and the cause is still unclear..Long non-coding RNA(lncRNA)is a key regulator of the underlying biological processes and pathogenesis of many diseases including ovarian cancer.Thus,this study aims to explore how LINC00936 influences ovarian cancer by regulating laminin alpha 3 chain gene(LAMA3)via microRNA-221-3p(miR-221-3p).Methods:Initially,the differentially expressed genes(DEGs)in ovarian cancer and miRNAs chip data related to ovarian cancer were identified.The expression of LINC00936、miR-221-3p and LAMA3 in ovarian cancer tissues and adjacent tissues were detected.Subsequently,ovarian cells transfected with overexpressed LINC00936、miR-221-3p or siRNA against miR-221-3p,or LAMA3 or siRNA against LAMA3 to reveal their role in cell proliferation、migration、invasion、angiogenesis and tumorigenesis of ovarian cancer.To further investigate the underlying mechanism of LINC00936 in ovarian cancer,the binding relationship between LINC00936 and miR-221-3p,and target relationship between miR-221-3p and LAMA3 were confirmed.Results:LINC00936 could upregulate LAMA3 by competitively binding to miR-221-3p.In addition,ovarian cancer cells exhibited downregulated LINC00936 and LAMA3 but upregulated miR-221-3p.Moreover,with the upregulation of LINC00936 and LAMA3,proliferation,migration,invasion and angiogenesis in ovarian cancer cells were inhibited.Conclusion:In summary,LINC00936 inhibits the progression of ovarian cancer cells by adsorbing miR-221-3p,which provides a new theoretical basis for cell proliferation,migration,invasion,angiogenesis and tumorigenesis of ovarian cancer.