Correlation Analysis Of The Effects Of Sociological Factors And DCAA,TCAA Exposure On The Metabolism Of Monoamine Neurotransmitters:A Cross-sectional Study In China

Objective: Monoamine neurotransmitters play an important role in regulating the nervous and cardiovascular systems.Sociological factors may impact the concentrations of monoamine neurotransmitters.But some conclusions are inconsistent in the existing researches.Sociological factors have obvious regional and sociological differences.Therefore,the object is to analyze the associations between sociological factors and the concentrations of three important monoamine neurotransmitters.Moreover,people are widely exposed to pollutants such as dichloroacetic acid(DCAA)and trichloroacetic acid(TCAA).The toxicity of DCAA and TCAA linked to the abnormal metabolisms of monoamine neurotransmitters.However,the associations between DCAA and TCAA exposure and the concentration changes of three monoamine neurotransmitters have not been studied to date.Therefore,we also examine the associations between DCAA and TCAA exposure and the three monoamine neurotransmitters.These may offer the sociological and etiological evidences for the future researches about the metabolic disorders of monoamine neurotransmitters.Methods: The subjects were selected from the person aged 18-55 who presented health examination in the Third Hospital of Hebei Medical University from October 2014 to September 2015.Concentrations of DCAA and TCAA in urine were measured by internal standard method.ELISA was used to detect the EPI,NE and 5-HT in urine.Moreover,general demographic data(gender,age,marital status,et,al.),personal behavioral factors(smoking,drinking,et,al.)and their health status were collected by questionnaire.Generalized linear model was used to analyze the correlation between DCAA and TCAA exposure and the change of EPI,NE and 5-HT.Multivariate linear model was used to analyze the associations of sociological factors with the three monoamine neurotransmitters.Results:1.The mean concentrations of DCAA and TCAA in urine were 4.98ug/L and 4.83ug/L,respectively;the mean concentrations of EPI,NE and 5-HT were 2075.44ng/L,257.80ng/L and 1526.33ng/L,respectively.2.The concentrations of DCAA and TCAA were significantly positive associated with the concentrations of urinary 5-HT.Compared to the reference group(the first quartile of DCAA),5-HT increased by 120.84ng/L(95% CI: 50.88,154.80)in the fourth quartile exposure group;Compared to the reference group(the first quartile of TCAA),5-HT increased by 79.48ng/L(95% CI: 27.70,131.25)in the second quartile exposure group.These associations of DCAA and TCAA with 5-HT remained significant in the sensitivity analyses.3.Among the sociological factors,the EPI concentration in urine is positively correlated with age.The EPI concentration in urine is negatively correlated with educational background.Subjests with high-education have a lower concentration in EPI than those with low-education.Finally,the 5-HT concentration in urine is positively correlated with triglycerides related to obesity.With the increased concentration of 5-HT in urine,the concentration of triglycerides increased.Conclusions: Among the sociological factors,age,educational level and triglyceride are the impact factors of the concentration changes of monoamine neurotransmitters in urine.There were positive correlations between DCAA and TCAA exposure and 5-HT concentration in urine,suggesting that DCAA and TCAA may be risk factors in 5-HT metabolism.Identifying these sociological factors and controlling the exposure levels of DCAA and TCAA could provide sociological and etiological evidences for future researches about health risk assessment of monoamine neurotransmitters.