Preventive And Protective Effects Of Alhagi Extract On Liver Injury Of Heatsroke Rats In Dry Heat Environment Of Desert

Object:To investigate the protective effect and mechanism of alhagi extract on liver injury injury of heatsroke rats in dry heat environment of desert Methods:Experiment 1: 40 SD rats were randomly divided into 4 groups: dry heat control group,Low dose alhagi extract pretreated group(L-alh group,100 mg/kg);Medium dose alhagi extract pretreated group(M-alh group,400mg/kg);High dose alhagi extract pretreated group(H-alh group,1000mg/kg)? The rats were intragastrically administered once a day for 1 week,according to the corresponding alhagi dose.On the 8th day,4 groups of SD rats were transferred to the northwest special environment artificial experimental chamber,and then fasted,water,and exposed for heat.Simulated dry heat environment {ambient temperature(41 ± 0.5)°C,relative humidity(10 ± 1)%)}.The experimental cabin surveillance video observation recorded the death time of the rats.Experiment 2 was carried out on the basis of the optimum dose of alhagi extract: 50 SD rats were randomly divided into 5 groups: normal temperature control group(NC group);dry heat control group(DHC group);Low dose alhagi extract pretreated group(L-alh group,100 mg/kg);Medium dose alhagi extract pretreated group(M-alh group,200mg/kg);High dose alhagi extract pretreated group(H-alh group,400mg/kg),except The NC and DHC groups were given normal saline for 1 week,and The other three groups were given the same method as the experiment one.On the 8th day,except for the NC group,the other rats were transferred to the northwest special environment artificial experimental chamber for fasting and water.At the 150 th minute after the start of the experiment,the SD rats reached the state of heat radiation.The rats were taken out from the experimental chamber to measure the rectal temperature.After abdominal anesthesia with 3% pentobarbital,the vena cava blood was removed and the liver tissue was taken and sacrificed.At the same time,the NC group took blood and killed it.Serum was detected for ALT,AST andLPS.Part of the liver tissue was taken for pathological section and electron microscopy.The remaining liver tissue was frozen in liquid nitrogen.CAT,MDA and SOD were detected according to the kit instructions.TNF-?,IL-1? and IL-6 levels were detected by ELISA,and HSP-70,NF-?B and Caspase-3 levels were detected by Western Blot.Results:The results of experiment 1 were as follows:(1)Compared with the dry heat control group,the survival time of the rats in the alhagi extract group was prolonged(P<0.05),and the optimal dose was 400 mg/kg.The results of the experiment 2:(1)successful replication of the heat stroke model,the core body temperature of the DHC group was significantly higher than that of the NC group(P<0.05)and greater than 42 °C,reaching the state of heat stroke.The anal temperature of the alhagi extract group was lower than that of the DHC group(P<0.05).(2)The liver pathological injury score of the DHC group was significantly higher than that of the NC group(P<0.05).The injury score of the alhagi extract group was lower than that of the DHC group(P<0.05).The difference were significant among alhagi extract group(P <0.05).(3)Under light microscope,the ultrachromatin structure of the liver nucleus of the DHC group gradually disappeared,and more heterochromatin-like structures appeared.The nuclear membrane pores were blurred and the nuclear membrane was incomplete.The mitochondrial raft structure was disordered and disappears.The cell membrane structure was destroyed and the cells were dissolved.(4)Compared with the NC group,the levels of ALT,AST and LPS in the DHC group were significantly higher(P<0.05).The levels of ALT,AST and LPS in the blood of the alhagi extract group were lower than those in the DHC group(P<0.05).The difference were significant among alhagi extract group(P <0.05).(5)Compared with the NC group,the content of CAT and SOD in the DHC group was lower than that in the NC group(P<0.05).The content of CAT and SOD in the liver tissue of the alhagi extract group was higher than that in the DHC group,and the MDA content was decreased(P<0.05),The difference were significant among alhagi extract group(P <0.05).(6)The levels of TNF-?,IL-1? and IL-6 in the liver of the DHC group were higher than the NC group(P<0.05).Compared with the DHC group,the levels of TNF-?,IL-1? and IL-6 of alhagi extract group were decreased(P<0.05).The difference were significant among alhagi extract group(P <0.05).(7)The DHC group compared with the NC group,the rat liver tissue NF-?B,Caspase-3,HSP The expression of-70 increased(P<0.05).Compared with DHC group,the expression of NF-?B and Caspase-3 decreased,while the expression of HSP-70 continued to increase(P<0.05).HSP between alhagi extracts.The difference were significant of HSP-70 among alhagi extract group(P <0.05).There was significant difference in the expression of NF-?B and Caspase-3 between L-alh and H-alh group(P<0.05).Conclusion:Alhagi extract can prolong the survival time of rats in dry heat environment.Alhagi extract has protective effecton on liver injury of heatsroke rats,this protective effect may be related to the Alhagi extract of Antioxidative stress,anti-inflammatory response,inhibition of NF-?B?Caspase-3 expression and enhancement of HSP-70 expression.