The Effect Of Mir-150 On The Occurrence And Development Of Diabetes In Mice Through NKT Cells And T Cells

Objectives To investigate the effect of mi R-150 on the occurrence and development of diabetes in mice through NKT cells and T cells.Methods Use of mi R-150 gene knockout mice(mi R-150 ko,mi R-150 knock-out)and C57BL/6J mice(Wild tupe,WT)as normal control,streptozocin(STZ)was injected into 150mg/kg/ mouse to prepare a diabetic mouse model,and Then observe the changes of blood glucose and diabetes incidence in mi R-150 KO and WT mice,as well as the effects on different immune cells,a-galcer 6ug/ mouse injection,and observe the effects on stzinduced diabetes incidence in WT and mi R-150 KO mice,as well as the changes in NKT and T cell cytokines.Twenty-four male mi R-150 and WT mice with matching ages(6-10 weeks)were selected and starved 12 h before injection,and the body weight of each mouse was recorded at about 20 g.Mi R-150 knockout mice and normal C57 mice were divided into four groups,6 mi R-150 KO and 6 WT mice in each group: STZ single group,STZ+α-galcer group,STZ+DMSO solvent control group and citric acid buffer solvent control group.Non-fasting blood glucose of the mouse tail blood sample was monitored.The day of injection was the 0th day,and the blood glucose value of the mice was recorded.After that,the blood glucose was measured and recorded every 3 days.1 Mouse genotype was identified by PCR.2 Real-time PCR was used to identify mi R-150 gene knockout.3 Flow cytometry was used to detect the changes of T cells,B cells,Treg and NKT cells in the spleen of mice,and the changes of IL-4,IFN-γand IL-17 produced in the T cells and NKT.4 The changes of islet decarboxylase autoantibody(GADA)and islet cell autoantibody(ICA)in mouse serum were detected by ELISA.Results 1 After 2 weeks of STZ injection,the diabetes success rate of mi R-150 KO mice was about 73.5%,and the diabetes success rate of WT wild mice was about 33.3%.The loss of mi R-150 resulted in the increased incidence of type 1 diabetes mellitus(T1DM)in mi R-150 KO mice.2 STZ and α-galcer mi R-150 KO mice diabetes is reduced,and incidence of WT mice have no obvious change,α-galcer significantly reduced the STZ induced happen T1 DM mi R-150 KO mice;3 STZ treatment increased NKT and decreased Treg cells in mi R-150 KO mice.4 After STZ induction,IFN-and IL-17 increased in the total T cells of mi R-150 KO mice.5 α-galcer decreased IL-17 expression in total T cells of mi R-150 KO mice.6 STZ treatment did not increase ICA and GADA autoantibody levels in mir-150 ko mice and C57BL/6J mice.Conclusions 1 The deletion of mir-150 promotes the occurrence of T1 DM,partly due to the decrease of Treg and the increase of IL-17 in T cells.Mi R-150 plays an important role in the pathogenesis and development of diabetes through NKT cells and T cells;2 The deletion of mi R-150 resulted in increased levels of ICA and GADA autoantibodies in mice,but did not cause increased blood glucose in mice.STZ treatment only increased ICA and GADA levels in WT mice,but had no effect on ICA and GADA levels in mir-150 mice;3 NKT specific cell activator a-galcer significantly reduced the occurrence of T1 DM in mi R-150 KO mice,which was related to the decreased expression of IL-17 in T cells.Figure eleven;Table seven;Reference one hundred and twenty-one...