Effect Of MiR-150 And NKT Cell Activation On The Development Of Type 1 Diabetes In Female Mice

Objectives To investigate the effect of miR-150 and NKT cell activation on the development of type 1 diabetes in female miceMethods Female mice(miR-150KO)and C57BL/6Jwild-type normal control mice(Mir--150 knockout)were used.Streptomycin(STZ)treatment was used to establish a type 1diabetes mellitus(TIDM)mouse model.NKT cells were activated by in-vivo injection ofα-GalCer.The number of NKT cells in spleens of two kinds of mice was determined by flow cytometry.Serological indexes of model mice were detected by automatic biochemical analyzer,and the main indexes were AST and ALT.The changes of pancreatic tissue in mice were detected by HE staining.Resμlts 1 miR-150 knockout mice in STZ-treated female mice were more likely to develop TIDM than C57BL/6J mice.2 In female miR-150 knockout mice and C57BL/6J mice,the number of visceral NKT cells increased and the prevalence of TIDM decreased.3 Compared with C57BL/6J mice,miR-150 knockout mice treated with STZ showed significantly higher mortality afterα-GalCer injection.4 In addition,the blood glucose of miR-150 knockout mice and C57BL/6J mice decreased significantly 12 h after the injection of α-GalCer.5 Activation of NKT cells by α-GalCer injection significantly increased AST and ALT in mice.The pancreatic damage of miR-150 knockout mice treated with STZ was more severe than that of C57BL/6J mice.miR-150 knockout mice treated with STZ and C57BL/6J mice were injected with α-GalCer.The pancreatic tissue destruction after activation of NKT cells was significantly reduced compared with STZ alone.Conclusions 1 The deletion of miR-150 gene resulted in an increased incidence of TIDM in female mice.2 α-GalCer activation of NKT cells inhibited TIDM in female miR-150K0 mice and C57BL/6J mice,the mechanism may be related to the reduction of pancreatic tissue destruction by α-GalCer.3 The simultaneous treatment of α-GalCer and STZ resulted in a significant increase in the death of female miR-150 KO mice,the mechanism of which may be related to the excessive decrease of blood glucose concentration and the significant increase of serum liver function indexes ALT and AST in miR-150 KO miceFigure eleven;Table six;Reference one hundred and thirty-eight.