| Objective In this experiment,using peanut meal as the main raw material,using solidstate fermentation method,using Bacillus natto and Monascus as fermenting strains,exploring the growth and lipid metabolism and intestinal metabolism of Peanut meal in high fat diet mice.The influence of the Tao provides a theoretical basis for the development and utilization of peanut meal resources.Methods Fifty male C57BL/6J mice were randomly divided into two groups: blank control group N(normal saline),model group M(normal saline),positive control group Y(atorvastatin 10 mg/kg),and low polypeptide.Dose group DL(polypeptide 10 mg/kg),polypeptide high dose group DH(polypeptide 20 mg/kg),10 in each group.Except for the blank control group,the other groups were fed with high-fat diet.The intragastric dose was 0.1 ml/20 g/d and the experiment lasted for 6 weeks.The food intake of the mice was recorded every day;the body weight and general condition of the mice were recorded every week;the blood was taken from the eyeball,and the indicators of lipid metabolism in the serum were detected by an automatic biochemical analyzer;the body length was measured after the mice were sacrificed;the mouse heart and liver were immediately removed.,kidney,spleen,weighing and calculating organ index;hospital sent to detect the relevant indicators in whole blood;kit method to detect leptin content in liver tissue;HE staining to observe liver lipid deposition;HE staining to observe small intestine villus injury The condition was detected by Western Blot.The expression of small intestine atresia protein ZO-1 and connexin Occludin was detected.The content of cecal was collected and 16 rs high-throughput sequencing was performed to explore the richness of the flora in the intestinal contents.Results After 6 weeks of different doses of peanut meal,the body weight change rate of M group was higher than that of other groups,and the body weight change rate of DH group was the lowest;DH group and DL group mice were longer than other groups.There was no significant difference in kidney index and cardiac index between the groups(P>0.05).The liver index of DH group was lower than that of M group,and the difference was statistically significant(P<0.05).The spleen index of DH group was higher than that of M group.The difference was statistically significant(P < 0.05).After 6 weeks of different doses of peanut meal,the contents of total cholesterol(TC),triglyceride(TG)and low density lipoprotein(LDL)in group M showed an increasing trend compared with group N(P<0.05).High-density lipoprotein(HDL)showed a decreasing trend(P<0.05),indicating that the high-fat model mice were successful;compared with M,the levels of TC,TG,and LDL in the DL and DH groups were decreased.The trend was(P<0.05),while the HDL content showed an upward trend(P<0.05).The platelet count(PLT)of the M group was significantly lower than that of the N group(P<0.05),while the mean platelet volume(MPV),platelet distribution width(PDW),and platelet count(PCT).There was no significant difference between the groups(P>0.05).After intervention with peanut meal,PLT was significantly higher than M group(P<0.05).The level of serum leptin in the peanut-peptone peptide intervention group was lower than that in the M group,and the serum leptin level in the DH group wasthe most significant(P<0.05).Compared with the M group,the number of fat vacuoles in the liver tissue of the peanut aphid peptide intervention group was reduced,the nuclear morphology was regular,the hepatic sinus was clearly visible,and the hepatic cord was closely arranged.After 6 weeks of different doses of peanut meal,the ileal villi of the peanut-peptone polypeptide intervention group increased,which reduced the hair loss.Compared with the model group,the peanut meal peptide intervention group significantly up-regulated the expression of ZO-1 and Occludin tight junction proteins(P<0.05).At the gate level,the top three species in group A(blank control group)and group B(model group)are Bacteroidetes,Firmicutes,Proteobacteria,and Group C(positive control group),group D(low-dose peanut meal polypeptide group)and group E(high-dose peanut meal polypeptide group)ranked in the top three,followed by Firmicutes,Bacteroides),Proteobacteria.Among them,the highest relative content of Bacteroidetes was model group(60.02%),blank control group was second(48.39%),positive control group was third(29.99%),and peanut meal polypeptide high dose group was the least(8.71%).The order of the thick-walled bacteria was high-to-low in the order of high-dose peanut meal,peanut low-dose group,positive control group,blank control group and model group.The relative content of Proteobacteria in each group was higher in the low-dose group(5.73%)and the blank control group(4.97%),followed by the high-dose group(3.13%)and the model group(2.41%).The least was positive control group(2.07%).The clustering curve of peanut high-dose peptide high-dose group has the widest width on the abscissa,and the curve on the ordinate is the most gradual,indicating that the abundance of the high-dose group of peanut meal is the highest;the clustering curve of the model group is on the abscissa.The upper transversal broadband is the narrowest,and the curve decreases steepest on the ordinate,indicating that its species diversity is the lowest among the groups,and the species diversity of the other groups is higher than the model group.Conclusion Peanut sputum polypeptide can promote the growth of mice.The growth of DH group is better than that of DL group.Peanut polypeptide can regulate the lipid metabolism of high fat diet mice.Peanut peptide can improve intestinal mucosa.Injury,increasing the abundance of the intestinal flora. |
PDF Full Text Request