The Expression And Heterogeneity Analysis Of PD-1/PD-L1 In Triple-negative Breast Cancer And The Clinical Significance Of TIL

Objective: To investigate the biological difference between Triple-Negative Breast Cancer(TNBC)and HER2 Positive Breast Cancer,the expression heterogeneity of PD-1 /PD-L1 in tumor cells,and the clinical significance of interstitial lymphocytes(TIL).Methods: 1,Collect and arrange University Affiliated Hospital of Qingdao between January 2011 and December 2012,receives and pathological specimens of breast cancer cases diagnosed by using immunohistochemical staining and immunofluorescence molecular hybridization method of specimens can be divided into TNBC and HER2 Positive Breast Cancer,randomly selected from TNBC 60 cases of breast cancer cases for the case group,the HER2 Positive breast cancer 60 cases as control group,analyzes its histological grade,tumor size,lymph node metastasis biology characteristics.2.Immunohistochemical staining of PD-1/PD-L1 was performed on pathological specimens,in which PD-1 was expressed in the cytoplasm of interstitial lymphocytes,PD-L1 was expressed in the membrane/cytoplasm of tumor cells and interstitial lymphocytes,and the expression of PD-1/PD-L1 in tumor cells was recorded and sorted out in the case group and the control group.3.HE sections were reviewed,and the percentage of lymphocyte infiltration(TIL)in tumor interstitium in HE sections of case group and control group was recorded and sorted.4.The expressions of biological characteristics and immune indexes in the two groups were compared,and the statistical values were calculated.5.The total survival time(OS)of patients in the case group and the control group was counted by telephone,letters and other means,and the OS of the two groups was compared to calculate the statistical value.Results: In TNBC,the percentages of patients aged less than 35,35 to 50,and over 50 were 1.8%,48.2%,and 50%,respectively.The corresponding percentages of HER2 Positive Breast Cancer were 8%,44%,and 48%.Among the tumor size stages in TNBC,there were 62.5% of TI stage,35.7% of T2 stage and 1.8% of T3+T4 stage,and 78% of TI stage,20% of T2 stage and 2% of T3+T4 stage in the HER2 Positive Breast Cancer.There were no significant differences between the two groups in age and tumor size stage(P = 0.319 、0.200).TNBC was more common in the left breast(64.3%),and histological grade III patients accounted for 67.9%,and no tumor metastasis was found in the ipsilateral regional lymph nodes,which was higher thanHER2 Positive Breast Cancer(P = 0.012,0.024 、0.017),with statistical significance.PD-1 / PD-L1 in TNBC.Conclusion: TNBC tends to occur in the left breast,with high histological grade of tumor cells,obvious heterogeneity,and low metastasis rate in ipsilateral regional lymph nodes.In addition,with the increase of PD-1/PD-L1 positive expression rate and TIL percentage in tumor cells,the better the prognosis of patients,the higher the five-year survival rate.In terms of age,TNBC did not show an obvious trend of younger age,and there was no significant difference between tumor size and stage and HER2 Positive Breast Cancer.Based on the high malignancy and low survival rate of TNBC,we suggest the detection of PD-1/PD-L1 and TIL,and actively explore the effective treatment of TNBC.